Construct: ORF TRCN0000471526
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006780.1_s317c1
- Derived from:
- ccsbBroadEn_13489
- DNA Barcode:
- CGTCGATTATTCTCCGCATATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPHP4 (261734)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471526
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017001002.1 | 94.3% | 92.9% | (many diffs) |
| 2 | human | 261734 | NPHP4 | nephrocystin 4 | XR_946605.1 | 88% | (many diffs) | |
| 3 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017000998.1 | 73% | 71.8% | (many diffs) |
| 4 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017000996.1 | 62.9% | 61.9% | (many diffs) |
| 5 | human | 261734 | NPHP4 | nephrocystin 4 | XM_011541214.1 | 62.9% | 61.8% | (many diffs) |
| 6 | human | 261734 | NPHP4 | nephrocystin 4 | XM_011541213.1 | 62.3% | 61.3% | (many diffs) |
| 7 | human | 261734 | NPHP4 | nephrocystin 4 | NM_015102.5 | 62.3% | 61.2% | (many diffs) |
| 8 | human | 261734 | NPHP4 | nephrocystin 4 | XM_006710563.3 | 62.3% | 61.2% | (many diffs) |
| 9 | human | 261734 | NPHP4 | nephrocystin 4 | XM_011541216.2 | 62.3% | 61.2% | (many diffs) |
| 10 | human | 261734 | NPHP4 | nephrocystin 4 | XM_011541217.2 | 62.3% | 61.2% | (many diffs) |
| 11 | human | 261734 | NPHP4 | nephrocystin 4 | XM_011541218.2 | 62.3% | 61.2% | (many diffs) |
| 12 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017000997.1 | 62.3% | 61.2% | (many diffs) |
| 13 | human | 261734 | NPHP4 | nephrocystin 4 | XM_011541215.1 | 59.8% | 58.8% | (many diffs) |
| 14 | human | 261734 | NPHP4 | nephrocystin 4 | XR_001737115.1 | 58.1% | (many diffs) | |
| 15 | human | 261734 | NPHP4 | nephrocystin 4 | XR_001737114.1 | 57.9% | (many diffs) | |
| 16 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017000999.1 | 50% | 48.9% | (many diffs) |
| 17 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017001000.2 | 50% | 48.9% | (many diffs) |
| 18 | human | 261734 | NPHP4 | nephrocystin 4 | NR_111987.2 | 49.6% | 1_220del;2954_5502del | |
| 19 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017001001.1 | 43.7% | 42.4% | (many diffs) |
| 20 | human | 261734 | NPHP4 | nephrocystin 4 | NM_001291594.2 | 26.7% | 24.2% | (many diffs) |
| 21 | human | 261734 | NPHP4 | nephrocystin 4 | NM_001291593.2 | 26.7% | 24.1% | (many diffs) |
| 22 | human | 261734 | NPHP4 | nephrocystin 4 | XM_017001003.1 | 26.7% | 24.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2799
- ORF length:
- 2733
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cgactggcac aggatcttca cccaaaacgt gcttgtccct ccccacccac 121 agagagcgcg ccagccttgg aaggaatcca cggcattcca gtgtgtcctc aagtggctgg 181 acggaccggt aattaggcag ggcgtgctgg aggtactgtc agaggttgaa tgccatctgc 241 gagtgtcttt ctttgatgtc acctaccggc acttctttgg gaggacgtgg aaaaccacag 301 tgaagccgac gaagagaccg ccgtccagga tcgtctttaa tgagcccttg tattttcaca 361 catccctaaa ccaccctcat atcgtggctg tggtggaagt ggtcgctgag ggcaagaaac 421 gggatgggag cctccagaca ttgtcctgtg ggtttggaat tcttcggatc ttcagcaacc 481 agccggactc tcctatctct gcttcccagg acaaaaggtt gcggctgtac catggcaccc 541 ccagagccct cctgcacccg cttctccagg accccgcaga gcaaaacaga cacatgaccc 601 tcattgagaa ctgcagcctg cagtacacgc tgaagccaca cccggccctg gagcctgcgt 661 tccaccttct tcctgagaac cttctggtgt ctggtctgca gcagatacct ggcctgcttc 721 cagctcatgg agaatccggc gacgctctcc gaaagcctcg cctccagaag cccatcacgg 781 ggcacttgga tgacttattc ttcaccctgt acccctccct ggagaagttt gaggaagagc 841 tgctggagct ccacgtccag gaccacttcc aggagggatg tggcccactg gacggtggtg 901 ccctggagat cctggagcgg cgcctgcgtg tgggcgtgca caatggtctg ggcttcgtgc 961 agaggccgca ggtcgttgta ctggtgcctg agatggatgt ggccttgacg cgctcagcta 1021 gcttcagcag gaaagtggtc tcctcttcca agaccagctc cgggagccaa gctctggttt 1081 tgagaagccg cctccgcctc ccagagatgg tcggccaccc tgcatttgcg gtcatcttcc 1141 agctggagta cgtgttcagc agccctgcag gagtggacgg caatgcagct tcggtcacct 1201 ctctgtccaa cctggcatgc atgcacatgg tccgctgggc tgtttggaac cccttgctgg 1261 aagctgattc tggaagggtg accctgcctc tgcagggtgg gatccagccc aacccctcgc 1321 actgtctggt ctacaaggta ccctcagcca gcatgagctc tgaagaggtg aagcaggtgg 1381 agtcgggtac actccggttc cagttctcgc tgggctcaga agaacacctg gatgcaccca 1441 cggagcctgt cagtggcccc aaagtggagc ggcggccttc caggaaacca cccacgtccc 1501 cttcgagccc gccagcgcca gtacctcgag ttctcgctgc cccgcagaac tcacctgtgg 1561 gaccagggtt gtcaatttcc cagctggcgg cctccccgcg gtccccgact cagcactgct 1621 tggccaggcc tacttcacag ctaccccatg gctctcaggc ctccccggcc caggcagagt 1681 tcccgttgga ggccggtatc tcccacctgg aagccgacct gagccagacc tccctggtcc 1741 tggaaacatc cattgccgaa cagttacagg agctgccgtt cacgcctttg catgccccta 1801 ttgttgtggg aacccagacc aggagctctg cagggcagcc ctcgagagcc tccatggtgc 1861 tcctgcagtc ctccggcttt cccgagattc tggatgccaa taaacagcca gccgaggctg 1921 tcagcgctac agaacctgtg acgtttaacc ctcagaagga agaatcagat tgtctacaaa 1981 gcaacgagat ggtgctacag tttcttgcct ttagcagagt ggcccaggac tgccgaggaa 2041 catcatggcc aaagactgtg tatttcacct tccagttcta ccgcttccca cccgcaacga 2101 cgccacgact gcagctggtc cagctggatg aggccggcca gcccagctct ggcgccctga 2161 cccacatcct cgtgcctgtg agcagagatg gcacctttga tgctgggtct cctggcttcc 2221 agctgaggta catggtgggc cctgggttcc tgaagccagg tgagcggcgc tgctttgccc 2281 gctacctggc cgtgcagacc ctgcagattg acgtctggga cggagactcc ctgctgctca 2341 tcggatctgc tgccgtccag atgaagcatc tcctccgcca aggccggccg gctgtgcagg 2401 cctcccacga gcttgaggtc gtggcaactg aataCGAGCA GGACAACATG GTGGTGAGTG 2461 GAGACATGCT GGGGTTTGGC CGCGTCAAGC CCATCGGCGT CCACTCGGTG GTGAAGGGCC 2521 GGCTGCACCT GACTTTGGCC AACGTGGGTC ACCCGTGTGA ACAGAAAGTG AGAGGTTGTA 2581 GCACATTGCC ACCGTCCAGA TCTCGGGTCA TCTCAAACGA TGGAGCCAGC CGCTTCTCTG 2641 GAGGCAGCCT CCTCACGACT GGAAGCTCAA GGCGCTGGGC TCTGCAGGCG ACTGTGCTGT 2701 TTGGAGAAGT GGGAACCCTG CCAGTGGCAT TTGTGTCTGG TTGGCTGCTC ATCTGCAAGG 2761 GAAGGAGAAA TGGAGAGAAG GTCAGGAGGA ACATAGACTG CCCAACTTTC TTGTACAAAG 2821 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2881 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2941 ACGACGTCGA TTATTCTCCG CATATCTAAC GCGTTAAGTC gacaatcaac ctctggatta 3001 caaaatttgt gaaagatt