Construct: ORF TRCN0000471546
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011617.1_s317c1
- Derived from:
- ccsbBroadEn_05124
- DNA Barcode:
- CACCTAGCTTTGATTGTGAGCTAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF114 (163071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471546
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 163071 | ZNF114 | zinc finger protein 114 | NM_001331097.1 | 100% | 100% | |
| 2 | human | 163071 | ZNF114 | zinc finger protein 114 | NM_001369811.1 | 100% | 100% | |
| 3 | human | 163071 | ZNF114 | zinc finger protein 114 | NM_001369812.1 | 100% | 100% | |
| 4 | human | 163071 | ZNF114 | zinc finger protein 114 | NM_153608.4 | 100% | 100% | |
| 5 | human | 163071 | ZNF114 | zinc finger protein 114 | NM_001331098.1 | 89.7% | 89.6% | (many diffs) |
| 6 | human | 163071 | ZNF114 | zinc finger protein 114 | XM_017026414.2 | 89.7% | 89.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1320
- ORF length:
- 1251
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccaggac tcggtgacct tcgcagacgt ggctgtgaac ttcaccaaag 121 aggagtggac cctgctggac ccagctcaga ggaatctcta cagagacgtg atgctggaaa 181 attctaggaa cttggcattc atagattggg caactccatg taaaaccaaa gacgcaaccc 241 ctcagccgga tattcttcct aaaagaacat ttcctgaagc caacagagtg tgtctcacga 301 gcatcagttc ccagcactcc acattaagag aagactggag atgccccaaa acagaggaac 361 cacacaggca gggggtgaat aatgtgaagc cacctgcagt tgcccctgag aaagatgaat 421 ctcctgttag catttgtgaa gatcatgaaa tgaggaacca ctctaaacct acctgcaggc 481 ttgtgccttc acagggagat tccataagac aatgtatcct aacacgtgac tcaagtattt 541 tcaagtataa tcctgtctta aacgatagtc aaaaaacaca tgaaaacaac gaagacgatg 601 gagtcttggg gtggaacatt cagtgggttc cgtgtgggag aaaaacagag ctgaaatcaa 661 gcacatggac tggcagtcag aacactgtgc atcatatacg tgatgaaatt gatacggggg 721 ccaacaggca ccagcggaat ccatttggaa aagctttccg tgaagacgga tcccttaggg 781 cacacaacac tcatgGTCGA GAGAAAATGT ATGATTTTAC TCAGTGCGAG AACACCTCCA 841 GAAATAACTC AATTCACGCC ATGCAGATGC AGTTGTATAC CGCAGAGACA AACAAGAAGG 901 ATTGTCAAAC TGGGGCAACC TCTGCCAACG CTCCAAATTC CGGTTCACAC AAGAGTCATT 961 GCACTGGAGA GAAAACCCAT AAATGCCCCG AATGTGGGAG AGCCTTTTTT TATCAGTCAT 1021 TCCTTATGAG ACATATGAAA ATTCACACTG GAGAGAAACC GTATGAATGT GGGAAATGTG 1081 GGAAAGCCTT TAGATATTCC TTACACCTTA ATAAACATTT AAGAAAGCAT GTTGTGCAGA 1141 AGAAGCCCTA CGAATGTGAA GAATGTGGGA AAGTCATTCG GGAGTCCTCA AAATATACAC 1201 ATATAAGGAG CCACACTGGA GAGAAACCCT ATAAATGTAA GACATGTGGA AAAGACTTTG 1261 CAAAGTCGTC AGGACTTAAA AAACATCTTA AGACTCACAA AGATGAGAAG CCCTGTGAAT 1321 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1381 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1441 TTTATATATC TTGTGGAAAG GACGACACCT AGCTTTGATT GTGAGCTAGA CGCGTTAAGT 1501 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt