Construct: ORF TRCN0000471551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013095.1_s317c1
- Derived from:
- ccsbBroadEn_05077
- DNA Barcode:
- GGAAAGGAGCGCGCCAACATACCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLAC8L1 (153770)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471551
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 153770 | PLAC8L1 | PLAC8 like 1 | NM_001029869.3 | 100% | 100% | |
| 2 | human | 153770 | PLAC8L1 | PLAC8 like 1 | XM_005268381.3 | 83.7% | 77.2% | (many diffs) |
| 3 | human | 153770 | PLAC8L1 | PLAC8 like 1 | XM_011537571.2 | 83.4% | 79% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 600
- ORF length:
- 531
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaattggttt ggaagtaact tcttcaggtg tcctgaggat ctttccttac 121 tcaacatata ctcaccTCTG TTGTCACACA TGTCATCTGA AGATGAACAT TTTATTTCCA 181 ACTTGAGAGG CCATGTACCA GCCAGCGCTG TGGTGAAGCA GCCTGTTCGG GGAGCCAGTG 241 GCAGGACGAC AATCACAGCA ATTGTCCAGA CTGGCGGGGG CTGGAGCACC GGTCTCTTCA 301 GTGTCTGCAG AGATAGGAGA ATTTGTTTCT GTGGTCTATT CTGTCCTATG TGTCTTGAGT 361 GTGACATCGC CAGGCATTAT GGAGAGTGTC TTTGTTGGCC GTTGTTACCT GGGTCCACCT 421 TTGCACTGAG AATTGGCACC AGGGAGAGAC ATAAAATACA GGGCACACTG TGTGAAGACT 481 GGCTGGCGGT GCACTGCTGT TGGGCTTTTT CCATCTGCCA GGTGGCCCGG GAACTCAAGA 541 TGAGGACCTC CCAAGTCTAT GAAATCTGTG CAGTCCCAAT GACTAAGGAC ACCCTGGTTT 601 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAGGAAA GGAGCGCGCC AACATACCAA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt