Construct: ORF TRCN0000471552
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010643.1_s317c1
- Derived from:
- ccsbBroadEn_14376
- DNA Barcode:
- TAAAGCTTCTGTTCTCGATATACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATA3 (130560)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471552
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 130560 | SPATA3 | spermatogenesis associated 3 | NM_139073.3 | 94.9% | 94.2% | 23G>N;85_111del;311T>C |
| 2 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XM_005246303.2 | 94.9% | 94.2% | 23G>N;85_111del;311T>C |
| 3 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XM_017003363.2 | 94.9% | 94.2% | 23G>N;85_111del;311T>C |
| 4 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_001738624.2 | 53.7% | (many diffs) | |
| 5 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_002959241.1 | 45.3% | (many diffs) | |
| 6 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_001738628.1 | 41.6% | (many diffs) | |
| 7 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_001738626.2 | 39.8% | (many diffs) | |
| 8 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_001738625.1 | 39.2% | (many diffs) | |
| 9 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_001738627.2 | 37.3% | (many diffs) | |
| 10 | human | 130560 | SPATA3 | spermatogenesis associated 3 | XR_922856.2 | 33.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 615
- ORF length:
- 549
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaaggtcaag aagaaaangt cagaggccag acgccaccga gactccacct 121 cccagcatgc tagctccaat TCCACCTCTC AGCAGCCTAG TCCTGAATCC ACACCACAGC 181 AGCCTAGCCC TGAATCCACA CCACAGCATT CCAGCCTTGA AACCACCTCC CGGCAGCCAG 241 CATTCCAAGC CCTTCCAGCA CCCGAAATCC GCCGCTCCTC TTGCTGCCTT TTATCTCCAG 301 ATGCTAACGT GAAGGCAGCC CCTCAATCCA GGAAAGCAGG GCCTCTGACT CGCGCCGGCC 361 CGCATTCCTG CTCCTGTGCC ACTTGCCCCT GCAGCTCCGC TTGCTGGCGT CGTCTGGGGC 421 TATGCCATAG CCGCATCTTC GATGTCCTTC TGCCTCGGGA CTGGCAGATG GCGCCAGGGA 481 GAGGACTCCC CAACCTGCTC ACCTTCTACA GAAAATCTTC AAGAAAACCC TCCAGTCATC 541 GTAACGCGTG TCCTCCAAGC CCTCGGAACT GTGGCTGTGG CTCTGGGGGC TCTAGGAGCT 601 GCCTACTACA TCACTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 661 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGCCCGTAA CTTGAAAGTA 721 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TAAAGCTTCT GTTCTCGATA 781 TACAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt