Construct: ORF TRCN0000471583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017694.1_s317c1
- Derived from:
- ccsbBroadEn_10242
- DNA Barcode:
- CACTTCCCCCAGGGAACAGAAATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUG1 (55000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55000 | TUG1 | taurine up-regulated 1 | NR_110493.1 | 5.3% | 1_2319del;2725_7528del | |
| 2 | human | 55000 | TUG1 | taurine up-regulated 1 | NR_002323.2 | 5.3% | 1_2319del;2725_7542del | |
| 3 | human | 55000 | TUG1 | taurine up-regulated 1 | NR_152871.1 | 5.2% | 1_2319del;2570_2694del;2850_7653del | |
| 4 | human | 55000 | TUG1 | taurine up-regulated 1 | NR_110492.1 | 4.9% | (many diffs) | |
| 5 | human | 55000 | TUG1 | taurine up-regulated 1 | NR_152869.1 | 4.4% | 1_2319del;2568_2569ins69;2656_7459del | |
| 6 | human | 55000 | TUG1 | taurine up-regulated 1 | NR_152870.1 | 4.4% | 1_2319del;2568_2569ins69;2656_7473del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGA CTCCAGATTT CCAGATTTTC AAGACCTGGA CCTGGAACCC GAAAGAGCTT 121 GTCACGATGC GGCAGGAACA CTGGAGGTAG ATTTTTTTTT ATTTTTGAAT TTTGGGACTG 181 TTGACCTTGC TGTGAGAAAA GAGACAACGA CTGAGCAAGC ACTACCACCA GCACTGTTAC 241 TGGGAATTAG AAGACCTGAG TTTCTGTCCA GACCCTCAGT GCAAACTGAG GATGCTCCAT 301 CCAAAGTGAA TTATGTCCTG TGCCTCCTGA TTGCTGAGTG TTCACCTGGA CCTTCTGACT 361 ACCTTCCCTG TGCTATTCCA TCAGCCTACA GACCTGGTAC CTGGATTTTT GCCCGAGATG 421 ATTCCTACCA CCTTACTACT GACGAAGACA CCCATTCCAG TGGACCACTG TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGACACT TCCCCCAGGG AACAGAAATG ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt