Construct: ORF TRCN0000471598
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014323.1_s317c1
- Derived from:
- ccsbBroadEn_10148
- DNA Barcode:
- CATTCCTGGTCCTATTTTAATCTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACBD7 (414149)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471598
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 414149 | ACBD7 | acyl-CoA binding domain con... | NM_001039844.3 | 99.6% | 100% | 153A>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 406
- ORF length:
- 264
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGGCCCTG CAGGCTGATT TTGACAGGGC TGCAGAAGAT 181 GTGAGGAAGC TGAAAGCAAG ACCAGATGAT GGAGAACTGA AAGAACTCTA TGGGCTTTAC 241 AAACAAGCAA TAGTTGGAGA CATTAATATT GCGTGTCCAG GAATGCTAGA TTTGAAAGGC 301 AAAGCCAAAT GGGAAGCATG GAACCTCAAA AAAGGGTTGT CGACGGAAGA TGCGACGAGT 361 GCCTATATTT CTAAAGCAAA GGAGCTGATA GAAAAATACG GAATTTACCC AACTTTCTTG 421 TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 481 TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT 541 GGAAAGGACG ACATTCCTGG TCCTATTTTA ATCTAACGCG TTAAGTCgac aatcaacctc 601 tggattacaa aatttgtgaa agatt