Construct: ORF TRCN0000471678
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015413.1_s317c1
- Derived from:
- ccsbBroadEn_07735
- DNA Barcode:
- TGTAATAGGATATTCGCACTTTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDT21 (11051)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471678
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11051 | NUDT21 | nudix hydrolase 21 | NM_007006.3 | 99.8% | 99.5% | 681C>A |
| 2 | mouse | 68219 | Nudt21 | nudix (nucleoside diphospha... | NM_026623.3 | 93.5% | 99.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtggtaccg cccaatcgct cgcagaccgg ctggccccgg ggggtcactc 121 agttcggcaa caagtacatc cagcagacga agcccctcac cctggagcgc accatcaacc 181 tgtaccctct taccaattat acttttggta caaaagagcc cctctacgag aaggacagct 241 ctgttgcagc cagatttcag cgcatgaggg aagaatttga taaaattgga atgaggagga 301 ctgtagaagg GGTTCTGATT GTACATGAGC ACCGGCTACC CCATGTGTTA CTGCTGCAGC 361 TGGGAACAAC TTTCTTCAAA CTACCTGGTG GTGAACTTAA CCCAGGAGAA GATGAAGTTG 421 AAGGACTAAA ACGCTTAATG ACAGAGATAC TGGGTCGTCA GGATGGAGTT TTGCAAGACT 481 GGGTCATTGA CGATTGCATT GGTAACTGGT GGAGACCAAA TTTTGAACCT CCTCAGTATC 541 CATATATTCC TGCACATATT ACAAAGCCTA AGGAACATAA GAAGTTGTTT CTGGTTCAGC 601 TTCAAGAAAA AGCCTTGTTT GCAGTCCCTA AAAATTACAA GCTGGTAGCT GCACCATTGT 661 TTGAATTGTA TGACAATGCA CCAGGATATG GACCCATCAT TTCTAGTCTC CCTCAGCTGT 721 TGAGCAGGTT CAATTTTATT TACAAATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGTAATAG 901 GATATTCGCA CTTTCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt