Construct: ORF TRCN0000471690
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009542.1_s317c1
- Derived from:
- ccsbBroadEn_03893
- DNA Barcode:
- CCAATCTGTACGAAATCTCAAGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPCS3 (60559)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471690
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60559 | SPCS3 | signal peptidase complex su... | NM_021928.4 | 100% | 100% | |
| 2 | mouse | 76687 | Spcs3 | signal peptidase complex su... | NM_029701.1 | 93.3% | 100% | (many diffs) |
| 3 | mouse | 76687 | Spcs3 | signal peptidase complex su... | XM_006509521.3 | 47% | 36% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 606
- ORF length:
- 540
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa cacggtgctg tcgcgggcga actcactgtt cgccttctcg ctgagcgtga 121 tggcggcgct caccttcggc tgcttcatca ccaccgcctT CAAAGACAGG AGCGTCCCGG 181 TGCGGCTGCA CGTCTCGCGG ATCATGCTAA AAAATGTAGA AGATTTCACT GGACCTAGAG 241 AAAGAAGTGA TCTGGGATTT ATCACATTTG ATATAACTGC TGATCTAGAG AATATATTTG 301 ATTGGAATGT TAAGCAGTTG TTTCTTTATT TATCAGCAGA ATATTCAACA AAAAATAATG 361 CTCTGAACCA AGTTGTCCTA TGGGACAAGA TTGTTTTGAG AGGTGATAAT CCGAAGCTGC 421 TGCTGAAAGA TATGAAAACA AAATATTTTT TCTTTGACGA TGGAAATGGT CTCAAGGGAA 481 ACAGGAATGT CACTTTGACC CTGTCTTGGA ACGTCGTACC AAATGCTGGA ATTCTACCTC 541 TTGTGACAGG ATCAGGACAC GTATCTGTCC CATTTCCAGA TACATATGAA ATAACGAAGA 601 GTTATTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 661 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 721 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCAATCTGT ACGAAATCTC AAGATACGCG 781 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt