Construct: ORF TRCN0000471712
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013208.1_s317c1
- Derived from:
- ccsbBroadEn_00936
- DNA Barcode:
- TATGGAGGAGTAAAAGCCGCCTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGALS1 (3956)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471712
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3956 | LGALS1 | galectin 1 | NM_002305.4 | 100% | 100% | |
| 2 | mouse | 16852 | Lgals1 | lectin, galactose binding, ... | NM_008495.2 | 85.9% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 471
- ORF length:
- 405
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttgtggtctg gtcgccagca acctgaatct caaacctgga gagtgccTTC 121 GAGTGCGAGG CGAGGTGGCT CCTGACGCTA AGAGCTTCGT GCTGAACCTG GGCAAAGACA 181 GCAACAACCT GTGCCTGCAC TTCAACCCTC GCTTCAACGC CCACGGCGAC GCCAACACCA 241 TCGTGTGCAA CAGCAAGGAC GGCGGGGCCT GGGGGACCGA GCAGCGGGAG GCTGTCTTTC 301 CCTTCCAGCC TGGAAGTGTT GCAGAGGTGT GCATCACCTT CGACCAGGCC AACCTGACCG 361 TCAAGCTGCC AGATGGATAC GAATTCAAGT TCCCCAACCG CCTCAACCTG GAGGCCATCA 421 ACTACATGGC AGCTGACGGT GACTTCAAGA TCAAATGTGT GGCCTTTGAC TGCCCAACTT 481 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 541 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 601 CTTGTGGAAA GGACGATATG GAGGAGTAAA AGCCGCCTTC ACGCGTTAAG TCgacaatca 661 acctctggat tacaaaattt gtgaaagatt