Construct: ORF TRCN0000471722
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016604.1_s317c1
- Derived from:
- ccsbBroadEn_03491
- DNA Barcode:
- ATCATTAAGAAGTTACTACCTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABITRAM (54942)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471722
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54942 | ABITRAM | actin binding transcription... | NM_017832.4 | 100% | 100% | |
| 2 | human | 54942 | ABITRAM | actin binding transcription... | XM_011518816.2 | 56.2% | 51.3% | (many diffs) |
| 3 | mouse | 230234 | Fam206a | family with sequence simila... | NM_001081420.1 | 81.3% | 78.7% | (many diffs) |
| 4 | mouse | 230234 | Fam206a | family with sequence simila... | XM_011250006.2 | 39.1% | 36.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc taccgagccc gaagccgcgg agccggtggt gccttcgctc gtggatcgat 121 acttcactcg ctggtacaaa ccggatgtca aaggaaaatt ttgtgaggac cactgtatac 181 tacagcactc taaccgaata tgtgtcatca cattggcaga atctcatcca gttcttcaaa 241 gtggaaaaaC AATTAAAAGC ATTTCCTATC AGATCAGTAC CAACTGTAGC AGACTTCAGA 301 ACAAGGTCTC TGGGAAATTT AAGCGGGGGG CACAGTTTCT AACAGAGCTT GCACCTCTCT 361 GTAAGATTTA CTGCTCAGAT GGTGAAGAAT ATACTGTGTC TAGTTGTGTT AGAGGACGTT 421 TGATGGAAGT GAATGAAAAC ATCCTCCATA AGCCATCTAT TCTTCAAGAA AAGCCATCTA 481 CTGAAGGCTA CATTGCAGTT GTGTTACCCA AATTTGAAGA AAGTAAAAGC ATAACAGAAG 541 GGTTACTGAC ACAAAAACAA TATGAAGAAG TCATGGTGAA ACGCATTAAT GCCACAACAG 601 CTACGTCATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 661 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 721 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAATCATT AAGAAGTTAC TACCTCTGAC 781 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt