Construct: ORF TRCN0000471809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012858.1_s317c1
- Derived from:
- ccsbBroadEn_07773
- DNA Barcode:
- CACTCAGATACTATACATTCATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDX20 (11218)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471809
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11218 | DDX20 | DEAD-box helicase 20 | NM_007204.5 | 99.7% | 99.7% | (many diffs) |
| 2 | mouse | 53975 | Ddx20 | DEAD (Asp-Glu-Ala-Asp) box ... | NM_017397.3 | 84.1% | 82.2% | (many diffs) |
| 3 | mouse | 53975 | Ddx20 | DEAD (Asp-Glu-Ala-Asp) box ... | XM_006501701.3 | 84.1% | 82.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2538
- ORF length:
- 2472
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcattt gaagcctcgg gagccttagc agcagtggcg actgctatgc 121 cggctgagca tgtggccgtg caggtcccgg ccccagagcc aacacccggg cctgtgagga 181 tcctgcggac cgctcaggat ctcagcagcc cgcggacccg cacgggggat gtgctgttgg 241 cggagccggc cgacttcgag tcactgctgc tttcgcggcc ggtgctggag gggctgcggg 301 cggccggctt cgagaggccc tcgccggtgc agctcaaggc catcccgctg gggcgctgcg 361 ggctcgattt aattgttcaa gctaaatctg gcaccgggaa aacctgtgtg ttctccacca 421 tagctttgga ctctcttgtt cttgaaaact taagtaccca gattttgatc ttggctccta 481 caagagaaat tgctgtacag atacattctg ttattacagc cattggaata aaaatggaag 541 gcttagagtg tcatgtcttt attggaggga ccccattatc acaagacaaa accagactta 601 aaaagtgtca tattgctgtt ggatctcctg gcagaattaa gcaactcata gaacttgact 661 acttgaaccc aggcagtata cgcctcttta ttcttgatga agcagataag cttttagaag 721 aaggcagctt ccaggagcaa ataaattgga tttattcttc cttgcctgcc agtaaacaga 781 tgctggcagt atcagctact tatcccgaat ttttggctaa tgctttgaca aagtacatga 841 gagatcccac ttttgtaaga ctgaattcca gtgatccaag tctcataggt ttgaagcagt 901 attacaaagt tgtcaattca taccctttgg cacataaggt ttttgaggaa aagactcagc 961 atttacagga actgttcagc agaattccat ttaatcaagc tttagtcttt tctaatttgc 1021 acagcagagc acaacatttg gctgatatcc tttcttctaa aggctttcct gctgagtgca 1081 tttcaggcaa tatgaatcag aatcagcgtc ttgatgctat ggctaaactg aagcattttc 1141 attgcagagt cctcatttcc acagatttga cttctcgtgg gattgatgct gagaaggtga 1201 atctggttgt aaatctggat gtaccattgg attgggagac atacatgcat cggattggga 1261 gagctggccg ttttggtaca ttggggctga cagtgaccta ctgttgccgg ggagaggaag 1321 aaaatatgat gatgagaatt gcccagaaat gtaatatcaa ccttctccct ttaccagatc 1381 ccattccttc tggtctgatg gaagaatgtg tggattggga tgtggaagtt aaagctgctg 1441 tgcatacata tggtatagca agtgtaccta accaaccctt aaaaaagcaa attcagaaaa 1501 tagagagaac ccttcaaatt cagaaagctc atggtgacca catggcttcc tctagaaata 1561 attctgtatc tggactatca gtcaaatcaa aaaataatac caaacaaaag cttcctgtga 1621 aaagccactc agaatgtgga atcatagaaa aagcaacgtc accaaaagaa ctgggctgtg 1681 acaggcaatc cgaagagcaa atgaagaatt ctgttcagac tcccgttgaa aactccacca 1741 acagtcagca ccaggtcaaa gaagctttac ctgtgtcact cccccagatt ccttgtctgt 1801 cttcctttaa aatccatcag ccatacacgt tgacttttgc tgaattggta gaggattatg 1861 aacattatat taaagagggg ttagagaaac ctgtggaaat catcaggcac tacacaggcc 1921 ctggggatca gactgtgaat cctcaaaatg gttttgtgag aaataaagtt actgaacaga 1981 gagtccctgt attggcaagt agtagccaat ctggagactc tgagagtgac agtgattctt 2041 acagctcaag aacctcttcc cagagcaaag gaaataagtc atacttggaa ggctcttctg 2101 ataatcagct gaaagactct gaatctacgc ctgtggatga tcgtatttct ttggaacaac 2161 caccaaatgg aagtgacacc cccaatccag agaaatatca agaaTCACCT GGAATCCAGA 2221 TGAAGACAAG ACTTAAAGAG GGGGCTAGCC AGAGAGCTAA GCAGAGCCGG AGAAACCTAC 2281 CCAGGCGGTC TTCCTTCAGA TTGCAGACTG GAGCCCAGGA AGATGATTGG TATGACTGTC 2341 ATAGGGAAAT ACGTCTGAGT TTTTCTGATA CCTATCAGGA TTATGAGGAG TACTGGAGAG 2401 CTTACTACAG GGCATGGCAA GAATATTATG CTGCCGCTTC TCATTCATAT TATTGGAATG 2461 CTCAGAGACA TCCAAGTTGG ATGGCAGCTT ATCACATGAA TACCATTTAT CTACAAGAAA 2521 TGATGCATAG TAACCAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2581 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2641 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACTCAG ATACTATACA 2701 TTCATATACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt