Construct: ORF TRCN0000471880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008336.1_s317c1
- Derived from:
- ccsbBroadEn_11115
- DNA Barcode:
- AATATTGCCAATCCGTTTCAAGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAA1 (6288)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471880
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6288 | SAA1 | serum amyloid A1 | NM_000331.5 | 98.6% | 97.5% | (many diffs) |
| 2 | human | 6288 | SAA1 | serum amyloid A1 | NM_001178006.2 | 98.6% | 97.5% | (many diffs) |
| 3 | human | 6288 | SAA1 | serum amyloid A1 | NM_199161.5 | 98.3% | 96.7% | (many diffs) |
| 4 | human | 6289 | SAA2 | serum amyloid A2 | NM_030754.5 | 96.4% | 91.8% | (many diffs) |
| 5 | human | 6289 | SAA2 | serum amyloid A2 | NM_001127380.3 | 66.1% | 60.6% | (many diffs) |
| 6 | human | 100528017 | SAA2-SAA4 | SAA2-SAA4 readthrough | NM_001199744.2 | 54.3% | 48% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 456
- ORF length:
- 366
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGAAGCTTCT CACGGGCCTG GTTTTCTGCT 121 CCTTGGTCCT GGGTGTCAGC AGCCGAAGCT TCTTTTCGTT CCTTGGCGAG GCTTTTGATG 181 GGGCTCGGGA CATGTGGAGA GCCTACTCTG ACATGAGAGA AGCCAATTAC ATCGGCTCAG 241 ACAAATACTT CCATGCTCGG GGGAACTATG ATGCTGCCAA AAGGGGACCT GGGGGTGTCT 301 GGGCTGCAGA AGCGATCAGC GATGCCAGAG AGAATATCCA GAGATTCTTT GGCCATGGTG 361 CGGAGGACTC ACTGGCCGAT CAGGCTGCCG ATGAATGGGG CAGGAGTGGC AAAGACCCCA 421 ATCACTTCCG ACCTGCTGGC CTGCCTGAGA AATACTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AAATATTGCC AATCCGTTTC AAGTCACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt