Construct: ORF TRCN0000471899
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005807.1_s317c1
- Derived from:
- ccsbBroadEn_13697
- DNA Barcode:
- TAAGCCACCCATGAAGTATGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC441242 (441242)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471899
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 102724014 | LOC102724014 | translation initiation fact... | XM_024452502.1 | 60.5% | 47.4% | (many diffs) |
| 2 | human | 102724052 | LOC102724052 | translation initiation fact... | XM_024452503.1 | 60.5% | 47.4% | (many diffs) |
| 3 | human | 441242 | LOC441242 | uncharacterized LOC441242 | NR_038378.4 | 44.7% | 1_897del;1356_1357ins43;1803_1978del | |
| 4 | human | 105379473 | LOC105379473 | uncharacterized LOC105379473 | XM_011546043.2 | 25.1% | 15.1% | (many diffs) |
| 5 | human | 105379474 | LOC105379474 | uncharacterized LOC105379474 | XM_011546044.2 | 25.1% | 15.1% | (many diffs) |
| 6 | human | 105379545 | LOC105379545 | uncharacterized LOC105379545 | XM_011546243.2 | 25.1% | 15.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1014
- ORF length:
- 948
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag agggccggac cagatggaaa ttcggggggg tccgtatctc ctgctcggtg 121 aggtcgttgg acacagcgca cttggtgccc agcccgcgca ggctgccaat ggagatgccg 181 atgagcttct ggagctgtcc gcactgctgc agccccgggc tggccggggc ccctgcgcca 241 ccctcagcgg ccgctgcatc acccccgcca ccgccctcct tcttctctcc catcgcctcc 301 gcgcgcagtg ccgccctatg caggccacag cggccgaggc agggagcccg gggctcgggc 361 gcctaggcaa ggaacccccg agccgggaga gctggaccgg gagcgcccct cggcgctgcc 421 cttgccagga cgccagtaga gctggcagcg gagtctgccg ctcccgccct cagagccgcg 481 gcggcgggag cagaaagccg cggcggcaaa aagccgcggc gacgggggca aaaagccgcg 541 gcggcgcagg caaaaagctt aGGCGGCGGG GGCAAAAAGC CGCGGCGGCG CAGGCAAAAA 601 GCTTAGGCGG CGGGGGCAAA AAGCCGCGGC GGCAAAAATG CGCGGCGGCG GGGCAAAAAG 661 CCGCGGCGGA AGAAAGCCGC CGGGGCGGGG GCAAAAAGCC GCGGCGGGGG GGACAAAAAG 721 CAGTGGGAGC TGGGGCAAAA AAACCACAAA AAGCCGCGGC GGCGGGGACC AAAACGCCGC 781 GGCGGCGGAG GCAAAAAGCT GCAAAAGCCG CGGTGTGGGG GCAGAAAGCC GCAGCGGGAG 841 AAAGTCTCAC TCCAAATTCC CTTGCTTCAG TAAGCTTCTC TAGGCCAATG TTCCTTCGGC 901 ATTACCCATT TTCTTCCTGT GAATGCAAAT CACATAAACA TGGAAGTACA TTTGACCATT 961 TTAATTGTTT AACATTCCAT GTCACACGAC CTTCCACAAT AGAATCAATG TATTACCCAA 1021 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1081 ATTCTACGTA GTAATGAACT AGCCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1141 TATCTTGTGG AAAGGACGAT AAGCCACCCA TGAAGTATGT GTTACGCGTT AAGTCgacaa 1201 tcaacctctg gattacaaaa tttgtgaaag att