Construct: ORF TRCN0000471951
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002196.1_s317c1
- Derived from:
- ccsbBroadEn_04888
- DNA Barcode:
- GTATCCCATAGCACGACACGTTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CMBL (134147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471951
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 134147 | CMBL | carboxymethylenebutenolidas... | NM_138809.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 801
- ORF length:
- 735
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc taacgaagct tatccttgtc cgtgtgacat tggccacaga cttgagtatg 121 gagggctagg ccgtgaagtt caagtcgagc acatcaaggc ttatgtcacc aaatcccccg 181 ttgatgcagg caaagctgtg attgtcattc aagatatatt tggctggcag ttgcccaata 241 ccagatatat agctgacatg atctcaggaa atggatacac aaccattgtt ccagacttct 301 ttgtagggca agagccttgg gacccctctg gcgactggtc tatcttccct GAGTGGCTGA 361 AAACAAGAAA TGCCCAGAAG ATCGATAGAG AGATCAGTGC TATCTTGAAG TATCTGAAAC 421 AACAGTGTCA TGCCCAGAAA ATTGGCATCG TGGGATTCTG CTGGGGTGGA ACTGCTGTCC 481 ATCATTTGAT GATGAAATAC TCAGAATTCA GGGCAGGGGT GTCCGTCTAT GGCATTGTCA 541 AGGATTCTGA AGACATTTAC AATTTAAAGA ACCCCACTTT GTTCATTTTT GCTGAAAATG 601 ATGTTGTGAT TCCACTCAAG GACGTATCTT TGCTGACTCA GAAGTTGAAA GAACACTGCA 661 AAGTTGAATA TCAAATTAAA ACATTTTCTG GGCAGACTCA TGGGTTCGTG CATCGGAAGA 721 GAGAAGATTG CTCACCTGCA GACAAGCCCT ACATTGACGA GGCCAGAAGG AATTTAATTG 781 AGTGGCTGAA CAAGTACATG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 841 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 901 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGTAT CCCATAGCAC 961 GACACGTTTG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt