Construct: ORF TRCN0000471952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000414.1_s317c1
- Derived from:
- ccsbBroadEn_03739
- DNA Barcode:
- TGATAGCCGAGTGACCACCTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NRIP3 (56675)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56675 | NRIP3 | nuclear receptor interactin... | NM_020645.3 | 100% | 100% | |
| 2 | human | 56675 | NRIP3 | nuclear receptor interactin... | XM_024448608.1 | 71.7% | 71.7% | 0_1ins204 |
| 3 | human | 56675 | NRIP3 | nuclear receptor interactin... | XM_024448609.1 | 71.7% | 71.7% | 0_1ins204 |
| 4 | human | 56675 | NRIP3 | nuclear receptor interactin... | XR_001747927.1 | 15.5% | 1_66del;487_488ins140;650_3621del | |
| 5 | mouse | 78593 | Nrip3 | nuclear receptor interactin... | NM_020610.1 | 91% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 789
- ORF length:
- 723
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ttactcaggg ctcctcactg agggcggccg caaggagacc gacatgcggg 121 aggcggcgtc actgcgacag cagcgccgga tgaagcaggc ggtgcagttc atccacaagg 181 actccgccga cctgctgccc ctggacggcc tcaagaagct gggctcgtcc aaggacatgc 241 aacctcataa tattctgcag aggcgcctca tggaaaccaa cctgtctaag ctccgaagcg 301 gtccccgtgt cccttgggcc tcTAAGACGA ACAAACTCAA TCAGGCTAAG TCTGAGGGGC 361 TAAAGAAGTC TGAGGAGGAT GACATGATTT TGGTTTCTTG CCAGTGTGCT GGAAAGGATG 421 TGAAAGCCTT GGTTGACACA GGCTGCCTAT ATAATCTCAT CTCTTTGGCC TGTGTGGACA 481 GATTGGGACT CAAGGAGCAT GTCAAATCCC ACAAGCATGA AGGAGAAAAG CTTTCTCTAC 541 CCCGGCATCT CAAAGTAGTG GGCCAGATTG AGCACCTAGT GATCACACTG GGCTCCCTCC 601 GCCTGGACTG CCCAGCAGCT GTGGTTGATG ACAATGAGAA AAACTTGTCC CTTGGTCTAC 661 AGACTCTCCG ATCTCTGAAG TGCATCATAA ACTTGGATAA GCACCGGCTG ATCATGGGGA 721 AGACAGACAA GGAAGAAATC CCTTTTGTGG AGACAGTCTC TTTGAATGAA GACAACACTT 781 CAGAAGCATA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 841 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 901 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATGATAG CCGAGTGACC ACCTTTTTAC 961 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt