Construct: ORF TRCN0000471983
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006775.1_s317c1
- Derived from:
- ccsbBroadEn_10412
- DNA Barcode:
- TATCGCCGTCTTAATTCACTTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471983
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100130950 | LOC100130950 | uncharacterized LOC100130950 | NR_152840.1 | 18.1% | 1_308del;612_1668del | |
| 2 | human | 100130950 | LOC100130950 | uncharacterized LOC100130950 | NR_034082.2 | 13.9% | 1_308del;612_2172del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 369
- ORF length:
- 303
- Sequence:
-
1 tcttccatTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGCA CCAAGGAAAG GCCATTTGTG GGGACATAGC AAGAAGGCAG CTGTCTGCAA 121 GCCAGGAGGA GAACCCTCAC CAGAAACCTC ACAGAAATCG GCCAACACCC TCATCTCGGG 181 CTTCCAGCTT CAGAACAACG AGAAAACAAA TTTCTGTTGT GTCCTGGTTC CCATTTGTAA 241 TTGGCACCCT AATGAAACCG TTTTCTGTAG CCCTGGAAAT GGCTGAAAAT GAGGGGAGGG 301 AAATAAAGTG CTATAATTCA GAGCATGGAG CACACCTTCC TGGTTCACCT GAGCTGGATT 361 CGGAGAGGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 421 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 481 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATATCGC CGTCTTAATT CACTTTTTAC 541 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt