Construct: ORF TRCN0000472000
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006677.1_s317c1
- Derived from:
- ccsbBroadEn_15530
- DNA Barcode:
- ACCCGCAGACAATTTCGTAGCATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PITPNA (5306)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472000
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5306 | PITPNA | phosphatidylinositol transf... | NM_006224.4 | 99.8% | 100% | 177C>T |
| 2 | human | 5306 | PITPNA | phosphatidylinositol transf... | XM_011523939.1 | 90.6% | 90.7% | 177C>T;297_298ins75 |
| 3 | mouse | 18738 | Pitpna | phosphatidylinositol transf... | NM_008850.2 | 93.2% | 98.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 876
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gctgctcaag gagtatcgag taatcctgcc tgtgtctgta gatgagtatc 121 aagtggggca gctgtattct gtggctgagg ccagtaaaaa tgaaacgggt ggtggcgaag 181 gcgtggaggt cctggtgaat gagccctacg agaaggacgg tgagaaaggc cagtacacac 241 ataagatcta ccacctgcag agcaaagtac ccacgtttgt tcgaatgctg gccccagagg 301 gagccctgaa tatacacgag aaagcctgga atgcttaccc ctactgcaga accgttatta 361 caaatgagta catgaaagaa gactttctga ttaaaattga aacctggcac aaaccagatc 421 ttggcacgca ggagaatgtg cataagctgg agcctgaggc gtggaaacac gtggaagccg 481 tatatataga cattgcagat cgaagccaag tgcTCAGCAA GGATTACAAG GCAGAGGAAG 541 ACCCAGCAAA ATTTAAATCT ATCAAAACAG GCCGAGGACC CTTGGGCCCC AATTGGAAGC 601 AAGAGCTTGT AAACCAGAAG GACTGCCCAT ATATGTGTGC ATACAAACTG GTGACCGTCA 661 AGTTCAAGTG GTGGGGCCTG CAGAACAAAG TGGAGAACTT CATCCATAAG CAAGAGAGGC 721 GTCTGTTTAC AAACTTCCAC AGGCAGCTGT TCTGTTGGCT CGATAAGTGG GTTGACCTGA 781 CCATGGACGA CATTCGAAGG ATGGAAGAAG AGACGAAGAG ACAGCTGGAT GAAATGAGAC 841 AAAAGGACCC AGTGAAAGGA ATGACAGCAG ATGACTACCC AACTTTCTTG TACAAAGTGG 901 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 961 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1021 AACCCGCAGA CAATTTCGTA GCATAACGCG TTAAGTCgac aatcaacctc tggattacaa 1081 aatttgtgaa agatt