Construct: ORF TRCN0000472017
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012022.1_s317c1
- Derived from:
- ccsbBroadEn_10433
- DNA Barcode:
- GGGCGTTTTTACCCATCACAATTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCN1 (3491)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472017
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3491 | CCN1 | cellular communication netw... | NM_001554.5 | 99.9% | 99.7% | 1141G>C |
| 2 | mouse | 16007 | Cyr61 | cysteine rich protein 61 | NM_010516.2 | 87.4% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ctcccgcatc gccagggcgc tcgccttagt cgtcaccctt ctccacttga 121 ccaggctggc gctctccacc tgccccgctg cctgccactg ccccctggag gcgcccaagt 181 gcgcgccggg agtcgggctg gtccgggacg gctgcggctg ctgtaaggtc tgcgccaagc 241 agctcaacga ggactgcagc aaaacgcagc cctgcgacca caccaagggg ctggaatgca 301 acttcggcgc cagctccacc gctctgaagg ggatctgcag agctcagtca gagggcagac 361 cctgtgaata taactccaga atctaccaaa acggggaaag tttccagccc aactgtaaac 421 atcagtgcac atgtattgat ggcgccgtgg gctgcattcc tctgtgtccc caagaactat 481 ctctccccaa cttgggctgt cccaaccctc ggctggtcaa agttaccggg cagtgctgcg 541 aggagtgggt ctgtgacgag gatagtatca aggaccccat ggaggaccag gacggcctcc 601 ttggcaagga gctgggattc gatgcctccg aggtggagtt gacgagaaac aatgaattga 661 ttgcagttgg aaaaggcagc tcactgaagc ggctccctgt ttttGGAATG GAGCCTCGCA 721 TCCTATACAA CCCTTTACAA GGCCAGAAAT GTATTGTTCA AACAACTTCA TGGTCCCAGT 781 GCTCAAAGAC CTGTGGAACT GGTATCTCCA CACGAGTTAC CAATGACAAC CCTGAGTGCC 841 GCCTTGTGAA AGAAACCCGG ATTTGTGAGG TGCGGCCTTG TGGACAGCCA GTGTACAGCA 901 GCCTGAAAAA GGGCAAGAAA TGCAGCAAGA CCAAGAAATC CCCCGAACCA GTCAGGTTTA 961 CTTACGCTGG ATGTTTGAGT GTGAAGAAAT ACCGGCCCAA GTACTGCGGT TCCTGCGTGG 1021 ACGGCCGATG CTGCACGCCC CAGCTGACCA GGACTGTGAA GATGCGGTTC CGCTGCGAAG 1081 ATGGGGAGAC ATTTTCCAAG AACGTCATGA TGATCCAGTC CTGCAAATGC AACTACAACT 1141 GCCCGCATGC CAATGAAGCA GCGTTTCCCT TCTACAGGCT GTTCAATGAC ATTCACAAAT 1201 TTAGGCACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1261 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1321 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGGGCGT TTTTACCCAT CACAATTCAC 1381 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt