Construct: ORF TRCN0000472021
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001118.1_s317c1
- Derived from:
- ccsbBroadEn_00347
- DNA Barcode:
- GTCGGTAAGCGTGCTTTAACCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CORT (1325)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472021
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1325 | CORT | cortistatin | NM_001302.5 | 67.7% | 67.7% | 0_1ins150 |
| 2 | human | 100526739 | CENPS-CORT | CENPS-CORT readthrough | NM_001243768.2 | 61.3% | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 534
- ORF length:
- 465
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtatagacac aaaaacagct ggagattggg cttaaaatac ccaccaagct 121 ccaaagaaga gacccaagtc ccCAAAACAT TGATTTCAGG GCTGCCAGGA AGGAAGAGCA 181 GCAGCAGGGT GGGAGAGAAG CTCCAGTCAG CCCACAAGAT GCCATTGTCC CCCGGCCTCC 241 TGCTGCTGCT GCTCTCCGGG GCCACGGCCA CCGCTGCCCT GCCCCTGGAG GGTGGCCCCA 301 CCGGCCGAGA CAGCGAGCAT ATGCAGGAAG CGGCAGGAAT AAGGAAAAGC AGCCTCCTGA 361 CTTTCCTCGC TTGGTGGTTT GAGTGGACCT CCCAGGCCAG TGCCGGGCCC CTCATAGGAG 421 AGGAAGCCCG GGAGGTGGCC AGGCGGCAGG AAGGCGCACC CCCCCAGCAA TCTGCGCGCC 481 GGGACAGAAT GCCCTGCAGG AACTTCTTCT GGAAGACCTT CTCCTCCTGC AAATTGCCAA 541 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 601 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 661 TATCTTGTGG AAAGGACGAG TCGGTAAGCG TGCTTTAACC CAAACGCGTT AAGTCgacaa 721 tcaacctctg gattacaaaa tttgtgaaag att