Construct: ORF TRCN0000472052
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006766.1_s317c1
- Derived from:
- ccsbBroadEn_05984
- DNA Barcode:
- GTTCGGCCGTCATGTCCTGTCTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CETP (1071)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472052
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1071 | CETP | cholesteryl ester transfer ... | NM_000078.3 | 99.9% | 99.7% | 1264G>A |
| 2 | human | 1071 | CETP | cholesteryl ester transfer ... | NM_001286085.2 | 87.7% | 87.6% | 746_747ins180;1084G>A |
| 3 | human | 1071 | CETP | cholesteryl ester transfer ... | XM_006721124.3 | 52.3% | 51.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggctgccaca gtcctgaccc tggccctgct gggcaatgcc catgcctgct 121 ccaaaggcac ctcgcacgag gcaggcatcg tgtgccgcat caccaagcct gccctcctgg 181 tgttgaacca cgagactgcc aaggtgatcc agaccgcctt ccagcgagcc agctacccag 241 atatcacggg cgagaaggcc atgatgctcc ttggccaagt caagtatggg ttgcacaaca 301 tccagatcag ccacttgtcc atcgccagca gccaggtgga gctggtggaa gccaagtcca 361 ttgatgtctc cattcagaac gtgtctgtgg tcttcaaggg gaccctgaag tatggctaca 421 ccactgcctg gtggctgggt attgatcagt ccattgactt cgagatcgac tctgccattg 481 acctccagat caacacacag ctgacctgtg actctggtag agtgcggacc gatgcccctg 541 actgctacct gtctttccat aagctgctcc tgcatctcca aggggagcga gagcctgggt 601 ggatcaagca gctgttcaca aatttcatct ccttcaccct gaagctggtc ctgaagggac 661 agatctgcaa agagatcaac gtcatctcta acatcatggc cgattttgtc cagacaaggg 721 ctgccagcat cctttcagat ggagacattg gggtggacat ttccctgaca ggtgatcccg 781 tcatcacagc ctcctacctg gagtcccatc acaagggtca tttcatctac aagaatgtct 841 cagaggacct ccccctcccc accttctcgc ccacactgct gggggactcc cgcatgctgt 901 acttctggtt ctctgagcga gtcttccact cgctggccaa ggtagctttc caggatggcc 961 gcctcatgct cagcctgatg ggagacgagt tcaaggcagt gctggagacc tggggcttca 1021 acaccaacca ggaaaTCTTC CAAGAGGTTG TCGGCGGCTT CCCCAGCCAG GCCCAAGTCA 1081 CCGTCCACTG CCTCAAGATG CCCAAGATCT CCTGCCAAAA CAAGGGAGTC GTGGTCAATT 1141 CTTCAGTGAT GGTGAAATTC CTCTTTCCAC GCCCAGACCA GCAACATTCT GTAGCTTACA 1201 CATTTGAAGA GGATATCGTG ACTACCGTCC AGGCCTCCTA TTCTAAGAAA AAGCTCTTCT 1261 TAAGCCTCTT GGATTTCCAG ATTACACCAA AGACTGTTTC CAACTTGACT GAGAGCAGCT 1321 CCGAGTCCAT CCAGAGCTTC CTGCAGTCAA TGATCACCGC TGTGGGCATC CCTGAGGTCA 1381 TGTCTCGGCT CGAGGTAGTG TTTACAGCCC TCATGAACAG CAAAGGCGTG AGCCTCTTCG 1441 ACATCATCAA CCCTGAGATT ATCACTCGAG ATGGCTTCCT GCTGCTGCAG ATGGACTTTG 1501 GCTTCCCTGA GCACCTGCTG GTGGATTTCC TCCAGAGCTT GAGCTACCCA ACTTTCTTGT 1561 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1621 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1681 GAAAGGACGA GTTCGGCCGT CATGTCCTGT CTTAACGCGT TAAGTCgaca atcaacctct 1741 ggattacaaa atttgtgaaa gatt