Construct: ORF TRCN0000472065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014297.1_s317c1
- Derived from:
- ccsbBroadEn_09666
- DNA Barcode:
- TCATCTTCCCTAGGCCTTATCGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC9B1 (150159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NM_139173.4 | 99.8% | 99.8% | 12A>T;99G>T |
| 2 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NM_001100874.3 | 88.9% | 86.1% | (many diffs) |
| 3 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NR_047513.2 | 76% | (many diffs) | |
| 4 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NR_047515.2 | 53.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1614
- ORF length:
- 1545
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcataccact gaatcaaaaa atgaacattt ggaggatgaa aacttccaaa 121 catctacaac tcctcagagt ctcattgatc ctaataatac tgcacatgaa gaaactaaaa 181 ctgtcttatc agatacagaa gaaataaaac cacagacaaa aaaggagaca tacatttctt 241 gtcctctaag aggagtattg aatgtaatta ttacaaatgg agttatactg tttgtgatat 301 ggtgtatgac ctggtcaatc ttaggctctg aagctctccc tggtggaaat ttatttgggt 361 tgttcattat tttttatagt gccattattg ggggaaaaat tttacaactc attagaatac 421 ctttagtgcc tccacttcca cctcttcttg ggatgttact ggctggtttt acgattagga 481 atgttccatt catcaatgaa catgtccatg ttcctaacac atggtcttca attttaagaa 541 gcattgccct taccattatt ctaataagag ctgggcttgg actcgatcca caggctttga 601 ggcatttgaa ggtcgtttgt ttcagattgg ctgtaggtcc atgccttatg gaggcaagtg 661 cagctgctgt tttttcacac ttcattatga aatttccctg gcaatgggca tttctattag 721 gttttgttct aggtgctgtc tctcctgctg ttgttgtccc ttacatgatg gtgctgcaag 781 aaaatggata tggtgttgag gaaggcattc caaccttatt aatggctgct agcagtatgg 841 atgacattct ggctatcact ggattcaata catgcttgag catagtcttt tcctcaggtg 901 gtatacttaa taacgccata gcctctataa ggaacgtatg tattagtctg ctggcaggaa 961 ttgttttggg attttttgtt cgatattttc caagtgaaga ccagaaaaaa cttacattga 1021 agagaggatt ccttgttttg actatgtgtg tttctgccgt cttaggcagc caacgtattg 1081 gtttacatgg atctggagga ttatgcacac tagtgttgag tttcattgca gggacaaaat 1141 ggTCCCAAGA AAAGATGAAA GTCCAAAAGA TTATTACGAC TGTATGGGAT ATTTTTCAAC 1201 CACTTCTTTT TGGTTTAGTT GGAGCAGAAG TATCTGTTTC ATCGCTTGAA TCAAATATTG 1261 TTGGCATATC TGTTGCCACT CTAAGTTTGG CATTATGTGT TCGAATTTTA ACCACATATC 1321 TATTGATGTG CTTTGCTGGT TTTAGTTTTA AGGAGAAAAT ATTTATTGCT TTAGCATGGA 1381 TGCCCAAAGC TACAGTACAG GCTGTGTTAG GTCCTCTGGC TCTAGAAACA GCAAGAGTCT 1441 CCGCACCCCA CTTGGAACCA TATGCGAAGG ATGTGATGAC AGTAGCATTT TTAGCCATCT 1501 TGATCACAGC TCCAAATGGA GCTCTACTTA TGGGCATTCT GGGGCCTAAA ATGCTTACAC 1561 GCCATTATGA TCCAAGCAAA ATAAAACTGC AGTTGTCAAC ATTAGAACAT CATTTGCCAA 1621 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1681 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1741 TATCTTGTGG AAAGGACGAT CATCTTCCCT AGGCCTTATC GATACGCGTT AAGTCgacaa 1801 tcaacctctg gattacaaaa tttgtgaaag att