Construct: ORF TRCN0000472091
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009437.1_s317c1
- Derived from:
- ccsbBroadEn_03205
- DNA Barcode:
- GCTATACCTGCCGCACAGGTGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFAF1 (51103)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472091
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51103 | NDUFAF1 | NADH:ubiquinone oxidoreduct... | NM_016013.4 | 100% | 100% | |
| 2 | human | 51103 | NDUFAF1 | NADH:ubiquinone oxidoreduct... | XM_006720555.3 | 100% | 100% | |
| 3 | human | 51103 | NDUFAF1 | NADH:ubiquinone oxidoreduct... | XM_011521658.1 | 100% | 100% | |
| 4 | human | 51103 | NDUFAF1 | NADH:ubiquinone oxidoreduct... | XM_011521659.3 | 89.6% | 85.6% | 832_833insAGATCTCTTCTAT;879_880ins89 |
| 5 | human | 51103 | NDUFAF1 | NADH:ubiquinone oxidoreduct... | XM_024449945.1 | 89.6% | 85.6% | 832_833insAGATCTCTTCTAT;879_880ins89 |
| 6 | human | 51103 | NDUFAF1 | NADH:ubiquinone oxidoreduct... | NR_045620.2 | 55.6% | 1_434del;1193_1446del;1670_1763del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tttggttcac aaattgctgc gtggtactta ttttctcaga aaattctcta 121 agccaacttc tgccttgtat ccatttttgg gtattcgctt tgcagagtat tccagtagtc 181 ttcagaaacc agtggcttct cctggcaaag cctcctcaca gaggaagact gaaggggatt 241 tgcaaggaga tcaccagaaa gaagttgctt tggatataac ttcttctgag gagaagcctg 301 atgttagttt cgataaagca attagagatg aagcaatata ccattttagg cttttgaagg 361 atgaaattgt ggatcattgg agaggaccgg aaggccaccc tctgcatgag gtcttgctgg 421 aacaagccaa ggttgtctgg caattccggg ggaaagaaga tttggataag tggacagtga 481 cttctgataa gacgattgga ggcagaagtg aagtgttttt gaaaatgggc aagaataacc 541 aaagtgcact gctatatgga actctgagct ctgaggcgcc TCAGGACGGG GAGTCTACCC 601 GAAGTGGGTA CTGTGCAATG ATATCCAGGA TTCCAAGGGG TGCTTTTGAG AGGAAGATGT 661 CTTACGATTG GTCCCAGTTC AATACTCTGT ATCTCCGTGT ACGTGGGGAT GGTCGGCCTT 721 GGATGGTGAA TATCAAGGAG GACACAGATT TCTTCCAGAG GACGAATCAG ATGTATAGTT 781 ACTTCATGTT CACCCGCGGG GGACCCTACT GGCAGGAGGT CAAGATTCCT TTTTCCAAAT 841 TTTTCTTCTC TAATCGAGGA AGAATCCGGG ATGTTCAGCA TGAGCTTCCG CTTGATAAGA 901 TCTCTTCTAT AGGATTCACC TTGGCTGATA AAGTGGATGG TCCATTCTTC CTGGAGATAG 961 ATTTTATTGG CGTGTTTACT GATCCAGCTC ATACAGAAGA ATTTGCCTAT GAAAATTCTC 1021 CAGAGCTTAA CCCAAGGCTT TTTAAATACC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCTATACC 1201 TGCCGCACAG GTGCACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt