Construct: ORF TRCN0000472093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013119.1_s317c1
- Derived from:
- ccsbBroadEn_03778
- DNA Barcode:
- GGTAACCGCTAGAGTTGGGTAATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ENTPD7 (57089)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57089 | ENTPD7 | ectonucleoside triphosphate... | NM_001349963.1 | 100% | 100% | |
| 2 | human | 57089 | ENTPD7 | ectonucleoside triphosphate... | NM_020354.5 | 100% | 100% | |
| 3 | human | 57089 | ENTPD7 | ectonucleoside triphosphate... | NM_001349962.2 | 99.5% | 99.3% | 4_10delCAGAACA;12_13insT |
| 4 | mouse | 93685 | Entpd7 | ectonucleoside triphosphate... | NM_053103.5 | 86.5% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1881
- ORF length:
- 1812
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctaggatc agtttttcct acctctgccc agcctcctgg tacttcactg 121 tgcccacagt gagtccattt ctccgtcagc gggtggcatt cctgggactc ttcttcatat 181 cctgtctcct tttacttatg ttaatcatag actttcgaca ttggagtgct tcattaccac 241 gagataggca atacgaaagg tatttggctc gagtagggga gcttgaagct actgacactg 301 aagacccaaa tctgaattat ggacttgttg ttgactgtgg cagcagtggt tcccggattt 361 ttgtttattt ctggccaaga cataatggga acccccatga cttgctggac atcaaacaga 421 tgagagaccg caacagccaa ccagtggtta aaaaaatcaa gccaggaatc tctgcaatgg 481 cagacactcc agaacatgcc agtgattacc ttcgtcctct gctgagcttt gctgctgctc 541 atgtgcctgt gaagaagcac aaggagaccc ctctttacat cctctgcaca gcaggcatga 601 ggcttctccc tgagaggaag cagttggcta tcttggctga cctagtgaaa gatttaccac 661 tggagtttga cttcctcttt tcacagtctc aagcagaagt gatctctggg aagcaggaag 721 gggtttatgc atggattgga atcaactttg ttttgggaag attcgaccac gaggatgaat 781 cagatgctga ggctacccag gaattggcag caggacggag aaggacagta gggatactgg 841 atatgggagg agcctctctc caaattgctt atgaagttcc tacctcaacc tctgtccttc 901 ctgcaaagca ggaagaagct gccaagatcc tgctggctga gttcaacctg ggctgtgatg 961 tgcaacacac tgaacacgtg tacagggttt atgtcacaac ttttctgggt ttcggaggca 1021 actttgcccg gcagcgctac gaagaccttg ttctgaatga aactcttaac aaaaacagat 1081 tgcttggtca gaagacaggt ctgagtcccg acaatccatt tctggatccc tgcctgccag 1141 tgggactcac agatgtggtg gagaggaaca gccaagtctt acatgtccga ggaagaggag 1201 actgggtgtc ttgtggggca atgctgagcc ccctgctggc tcgctccaac accagccagg 1261 cctcactcaa tggcatatat caatcgccta ttgacttcaa caacagcgag ttctacggct 1321 tctctgagtt tttttattgt acagaggatg tgttgcgcat tggtggccgc taccatgggc 1381 caacatttgc caaggctgcT CAGGATTACT GTGGCATGGC TTGGTCGGTA CTAACTCAGA 1441 GATTCAAGAA TGGCCTCTTT TCATCACATG CAGATGAGCA TCGACTCAAA TATCAGTGTT 1501 TTAAATCGGC TTGGATGTAC CAAGTCTTAC ATGAAGGATT CCACTTTCCC TATGACTACC 1561 CAAACCTGCG GACAGCCCAG CTGGTGTATG ACCGAGAGGT TCAGTGGACG CTGGGAGCCA 1621 TTCTATATAA AACACGATTC TTACCACTCA GGGATCTTCG GCAGGAAGGT GTCCGACAAG 1681 CCCATGGTAG CTGGTTCCGT CTCTCCTTTG TATACAACCA CTATCTCTTC TTTGCCTGTA 1741 TCCTGGTGGT GCTACTGGCC ATCTTCCTAT ACCTTCTGCG GCTACGCCGA ATTCACCACC 1801 GACAAACACG AGCCTCAGCT CCATTGGACT TGCTGTGGCT TGAAGAGGTG GTGCCCATGA 1861 TGGGAGTACA GGTGGGGCCG TTGCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1921 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1981 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGGTA ACCGCTAGAG 2041 TTGGGTAATC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt