Construct: ORF TRCN0000472098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011808.1_s317c1
- Derived from:
- ccsbBroadEn_05214
- DNA Barcode:
- CAGGCACCACGTCTACAAGCTCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VMA21 (203547)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472098
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 203547 | VMA21 | vacuolar ATPase assembly fa... | NM_001017980.3 | 100% | 100% | |
| 2 | human | 203547 | VMA21 | vacuolar ATPase assembly fa... | NM_001363810.1 | 62% | 55.6% | (many diffs) |
| 3 | mouse | 67048 | Vma21 | VMA21 vacuolar H+-ATPase ho... | NM_001081356.3 | 86.7% | 96% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 93
- ORF end:
- 396
- ORF length:
- 303
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA CCATGGAGCG CCCGGATAAG GCGGCGCTGA 121 ACGCACTGCA GCCTCCTGAG TTCAGAAATG AAAGCTCATT AGCATCTACA CTGAAGACGC 181 TCCTGTTCTT CACAGCTTTA ATGATCACTG TTCCTATTGG GTTATATTTC ACAACTAAAT 241 CTTACATATT TGAAGGCGCC CTTGGGATGT CCAATAGGGA CAGCTATTTT TACGCTGCTA 301 TTGTTGCAGT GGTCGCCGTC CATGTGGTGC TGGCCCTCTT TGTGTATGTG GCCTGGAATG 361 AAGGCTCACG ACAGTGGCGT GAAGGCAAAC AGGATTTGCC AACTTTCTTG TACAAAGTGG 421 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 481 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 541 ACAGGCACCA CGTCTACAAG CTCCAACGCG TTAAGTCgac aatcaacctc tggattacaa 601 aatttgtgaa agatt