Construct: ORF TRCN0000472150
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005594.1_s317c1
- Derived from:
- ccsbBroadEn_11313
- DNA Barcode:
- ATCTCTTCGCCGTGCTGACATTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APLN (8862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472150
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8862 | APLN | apelin | NM_017413.5 | 24.8% | 11.7% | 1_112del;231_232ins247 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 456
- ORF length:
- 366
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGTCCGCCAC CTGGTGCAGC CCAGAGGGTC 121 AAGGAATGGG CCAGGGCCCT GGCAGGGAGG TCGGAGGAAA TTCCGCCGCC AGCGGCCCCG 181 CCTCTCCCAT AAGGGACCCA TGCCTTTCTG AAGCAGGACT GAAGGGGCCC CCAAGTGCCC 241 ACCCCCGGCG GTTATGTCTC CTCCATAGAT TGGTCTGCTT CTCTGGAGGC CTCACGTCCA 301 TTCAGCTCTC ACCTCGCACC TGCTGTAGCC ACCAGTGGGC CCAGCTCTTC TCACCTGCCT 361 GCTTCCCCCA GTGGCGTGCT CCTGGCTGTA GTTTGGATGA TTCCCGTTCT CTCACAAGAA 421 TCCGTCCAGT CCATCTTCCT GGCCCCTCCC TGGACTGCCC AACTTTCTTG TACAAAGTGG 481 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 541 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 601 AATCTCTTCG CCGTGCTGAC ATTATACGCG TTAAGTCgac aatcaacctc tggattacaa 661 aatttgtgaa agatt