Construct: ORF TRCN0000472168
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005494.1_s317c1
- Derived from:
- ccsbBroadEn_12804
- DNA Barcode:
- CCAAGGATTTAGAGCCGCTATCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DDI2 (84301)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472168
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84301 | DDI2 | DNA damage inducible 1 homo... | NM_032341.5 | 52.8% | 52.8% | 634_1197del |
| 2 | mouse | 68817 | Ddi2 | DNA-damage inducible protein 2 | NM_001017966.2 | 47.7% | 50.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gctcaccgtg tactgtgtgc ggagggacct ctccgaggtg accttttccc 121 tccaggtcga cgccgacttc gagctgcaca acttccgcgc gctgtgcgag ctcgagtctg 181 gcatccccgc agccgagagc cagatcGTCT ATGCGGAAAG ACCTCTCACA GACAACCACA 241 GATCATTGGC TTCTTATGGC TTGAAAGATG GGGACGTTGT GATTTTACGA CAGAAGGAGA 301 ATGCAGACCC TCGACCTCCA GTGCAGTTCC CAAACTTACC CCGAATAGAT TTCAGTAGTA 361 TAGCTGTGCC TGGCACATCA AGTCCCCGGC AGCGCCAGCC ACCAGGAACA CAGCAGTCCC 421 ACTCATCTCC TGGAGAAATA ACTTCATCTC CTCAGGGCTT GGACAATCCA GCCTTGCTCC 481 GAGATATGTT GCTGGCCAAC CCGCATGAGC TGTCCTTGCT GAAGGAACGC AATCCACCCC 541 TGGCAGAAGC TCTGCTCAGT GGAGACCTTG AGAAATTTTC TAGAGTCCTG GTGGAGCAGC 601 AGCAGGACCG AGCCCGGAGA GAGCAAGAAA GGATTCGTCT GTTTTCTGCT GATCCCTTTG 661 ACCTTGAAGC TCAGGCAAAG ATAGAAGAAG ATATAAGGTA CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGACCAAGG ATTTAGAGCC GCTATCACAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt