Construct: ORF TRCN0000472208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016595.1_s317c1
- Derived from:
- ccsbBroadEn_05868
- DNA Barcode:
- TCTAAATCAGCCCGCTCTACAAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5F1C (509)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 509 | ATP5F1C | ATP synthase F1 subunit gamma | NM_001001973.3 | 99.8% | 99.6% | 133A>G |
| 2 | human | 509 | ATP5F1C | ATP synthase F1 subunit gamma | NM_005174.3 | 99.5% | 99.3% | 133A>G;891_892insGAT |
| 3 | human | 509 | ATP5F1C | ATP synthase F1 subunit gamma | NM_001320886.1 | 84.2% | 84.2% | 0_1ins141 |
| 4 | human | 509 | ATP5F1C | ATP synthase F1 subunit gamma | XM_017016290.1 | 83.8% | 83.8% | 0_1ins141;750_751insGAT |
| 5 | mouse | 11949 | Atp5c1 | ATP synthase, H+ transporti... | NM_020615.4 | 87.3% | 90.9% | (many diffs) |
| 6 | mouse | 11949 | Atp5c1 | ATP synthase, H+ transporti... | XM_006497310.2 | 87% | 90.6% | (many diffs) |
| 7 | mouse | 11949 | Atp5c1 | ATP synthase, H+ transporti... | NM_001112738.1 | 80.6% | 84.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 960
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ctctcgcgcg ggtgtcgctg ggctgtcggc ctggaccttg cagccgcaat 121 ggattcaagt tcgaaatatg gcaactttga aagatatcac caggagacta aagtccatca 181 aaaacatcca gaaaattgcc aagtctatga aaatggtagc ggcagcaaaa tatgcccgag 241 ctgagagaga gctgaaacca gctcgaatat atggattggg atctttagct ctgtatgaaa 301 aagctgatat caaggggcct gaagacaaga agaaacacct ccttattggt gtgtcctcag 361 atcgaggact gtgtggtgct attcattcct ccattgctaa acagatgaaa agcgaggttg 421 ctacactaac agcagctggg aaagaagtta tgcttgttgg aattggtgac aaaatcagag 481 gcatacttta taggactcat tctgaccagt ttctggtggc attcaaagaa gtgggaagaa 541 agccccccac ttttggagat gcgtcagtca ttgcccttga attactaaat tctggatatg 601 aatttgatga aggctccatc aTCTTTAATA AATTCAGGTC TGTCATCTCC TATAAGACAG 661 AAGAAAAGCC CATCTTTTCC CTTAATACCG TTGCAAGTGC TGACAGCATG AGTATCTATG 721 ACGATATTGA TGCTGACGTG CTGCAAAATT ACCAAGAATA CAATCTGGCC AACATCATCT 781 ACTACTCTCT GAAGGAGTCC ACCACTAGTG AGCAGAGTGC CAGGATGACA GCCATGGACA 841 ATGCCAGCAA GAATGCTTCT GAGATGATTG ACAAATTGAC ATTGACATTC AACCGTACCC 901 GCCAAGCTGT CATCACAAAA GAGTTGATTG AAATTATCTC TGGTGCTGCA GCTCTGGATT 961 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1021 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1081 TTTATATATC TTGTGGAAAG GACGATCTAA ATCAGCCCGC TCTACAAAAA CGCGTTAAGT 1141 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt