Construct: ORF TRCN0000472209
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008267.1_s317c1
- Derived from:
- ccsbBroadEn_00638
- DNA Barcode:
- CTAATATCCGGAGGAGTTTGAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GIP (2695)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472209
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2695 | GIP | gastric inhibitory polypeptide | NM_004123.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 525
- ORF length:
- 459
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggccacgaag acctttgctc tgctgctgct gtccctgttc cTGGCAGTGG 121 GACTAGGAGA GAAGAAAGAG GGTCACTTCA GCGCTCTCCC CTCCCTGCCT GTTGGATCTC 181 ATGCTAAGGT GAGCAGCCCT CAACCTCGAG GCCCCAGGTA CGCGGAAGGG ACTTTCATCA 241 GTGACTACAG TATTGCCATG GACAAGATTC ACCAACAAGA CTTTGTGAAC TGGCTGCTGG 301 CCCAAAAGGG GAAGAAGAAT GACTGGAAAC ACAACATCAC CCAGAGGGAG GCTCGGGCGC 361 TGGAGCTGGC CAGTCAAGCT AATAGGAAGG AGGAGGAGGC AGTGGAGCCA CAGAGCTCCC 421 CAGCCAAGAA CCCCAGCGAT GAAGATTTGC TGCGGGACTT GCTGATTCAA GAGCTGTTGG 481 CCTGCTTGCT GGATCAGACA AACCTCTGCA GGCTCAGGTC TCGGTGCCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA CTAATATCCG GAGGAGTTTG AAATACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt