Construct: ORF TRCN0000472250
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004285.1_s317c1
- Derived from:
- ccsbBroadEn_07597
- DNA Barcode:
- GTTGTCTACCTCAAAAATTGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RACK1 (10399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472250
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10399 | RACK1 | receptor for activated C ki... | NM_006098.5 | 99.8% | 100% | 753C>T |
| 2 | mouse | 14694 | Rack1 | receptor for activated C ki... | NM_008143.3 | 90.7% | 100% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1017
- ORF length:
- 951
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgagcagatg acccttcgtg gcaccctcaa gggccacaac ggctgggtaa 121 cccagatcgc tactaccccg cagttcccgg acatgatcct ctccgcctct cgagataaga 181 ccatcatcat gtggaaactg accagggatg agaccaacta tggaattcca cagcgtgctc 241 tgcggggtca ctcccacttt gttagtgatg tggttatctc ctcagatggc cagtttgccc 301 tctcaggctc ctgggatgga accctgcgcc tctgggatct cacaacgggc accaccacga 361 ggcgatttgt gggccatacc aaggatgtgc tgagtgtggc cttctcctct gacaaccggc 421 agattgtctc tggatctcga gataaaacca tcaagctatg gaataccctg ggtgtgtgca 481 aatacactgt ccaggatgag agccactcag agtgggtgtc ttgtgtccgc ttctcgccca 541 acagcagcaa ccctatcatc gtctcctgtg gctgggacaa gctggtcaag gtatggaacc 601 tggctaactg caagctgaag accaaccaca ttGGCCACAC AGGCTATCTG AACACGGTGA 661 CTGTCTCTCC AGATGGATCC CTCTGTGCTT CTGGAGGCAA GGATGGCCAG GCCATGTTAT 721 GGGATCTCAA CGAAGGCAAA CACCTTTACA CGCTAGATGG TGGGGACATC ATCAACGCCC 781 TGTGCTTCAG CCCTAACCGC TACTGGCTGT GTGCTGCTAC AGGCCCCAGC ATCAAGATCT 841 GGGATTTAGA GGGAAAGATC ATTGTAGATG AACTGAAGCA AGAAGTTATC AGTACCAGCA 901 GCAAGGCAGA ACCACCCCAG TGCACCTCCC TGGCCTGGTC TGCTGATGGC CAGACTCTGT 961 TTGCTGGCTA CACGGACAAC CTGGTGCGAG TGTGGCAGGT GACCATTGGC ACACGCTACC 1021 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1081 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1141 ATATATCTTG TGGAAAGGAC GAGTTGTCTA CCTCAAAAAT TGTTGAACGC GTTAAGTCga 1201 caatcaacct ctggattaca aaatttgtga aagatt