Construct: ORF TRCN0000472311
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011369.1_s317c1
- Derived from:
- ccsbBroadEn_05549
- DNA Barcode:
- CACCATAGATATGAGTTCCGACTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GJB7 (375519)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472311
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375519 | GJB7 | gap junction protein beta 7 | NM_198568.3 | 100% | 100% | |
| 2 | human | 375519 | GJB7 | gap junction protein beta 7 | XM_005248716.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttggatgttc ctcagagatc tcctgagtgg agtaaataaa tactccactg 121 ggactggatg gatttggctg gctgtcgtgt ttgtcttccg tttgctggtc tacatggtgg 181 cagcagagca cgtgtggaaa gatgagcaga aagagtttga gtgcaacagt agacagcccg 241 gttgcaaaaa tgtgtgtttt gatgacttct tccccatttc ccaagtcaga ctttgggCCT 301 TACAACTGAT AATGGTCTCC ACACCTTCAC TTCTGGTGGT TTTACATGTA GCCTATCATG 361 AGGGTAGAGA GAAAAGGCAC AGAAAGAAAC TCTATGTCAG CCCAGGTACA ATGGATGGGG 421 GCCTATGGTA CGCTTATCTT ATCAGCCTCA TTGTTAAAAC TGGTTTTGAA ATTGGCTTCC 481 TTGTTTTATT TTATAAGCTA TATGATGGCT TTAGTGTTCC CTACCTTATA AAGTGTGATT 541 TGAAGCCTTG TCCCAACACT GTGGACTGCT TCATCTCCAA ACCCACTGAG AAGACGATCT 601 TCATCCTCTT CTTGGTCATC ACCTCATGCT TGTGTATTGT GTTGAATTTC ATTGAACTGA 661 GTTTTTTGGT TCTCAAGTGC TTTATTAAGT GCTGTCTCCA AAAATATTTA AAAAAACCTC 721 AAGTCCTCAG TGTGTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CACCATAGAT ATGAGTTCCG 901 ACTCACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt