Construct: ORF TRCN0000472326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011628.1_s317c1
- Derived from:
- ccsbBroadEn_11678
- DNA Barcode:
- GCACCCCTCCGAGGTTCTTCGGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MRPS27 (23107)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472326
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23107 | MRPS27 | mitochondrial ribosomal pro... | NM_001286751.2 | 46.8% | 46.9% | 1_570del;855T>C |
| 2 | human | 23107 | MRPS27 | mitochondrial ribosomal pro... | NM_015084.3 | 40.4% | 40.5% | 1_738del;1023T>C |
| 3 | human | 23107 | MRPS27 | mitochondrial ribosomal pro... | NM_001286748.2 | 39.1% | 39.2% | 1_780del;1065T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tctgatatgg aaaccaggct accttgacag agcccttcaa gtgatggaga 121 aagtggctgc ctccccagaa gacataaAGC TGTGTAGAGA AGCGCTCGAT GTGCTGGGTG 181 CAGTGCTGAA GGCTCTGACT TCAGCTGATG GGGCTTCAGA GGAGCAGTCC CAAAATGATG 241 AAGACAACCA GGGGTCAGAA AAACTGGTGG AGCAGTTAGA CATCGAGGAA ACAGAGCAGT 301 CCAAGCTTCC TCAATACCTG GAACGATTTA AGGCCTTACA TTCTAAGCTC CAAGCTCTGG 361 GCAAAATTGA GTCAGAAGGT CTTTTAAGTC TGACCACCCA GCTTGTCAAG GAAAAACTCT 421 CCACCTGTGA AGCAGAGGAC ATCGCCACCT ATGAGCAGAA TCTGCAGCAG TGGCATCTAG 481 ACCTTGTACA GTTGATCCAG AGAGAACAGC AACAGAGGGA GCAAGCGAAG CAGGAGTACC 541 AGGCTCAGAA AGCAGCAAAG GCATCTGCCT ACCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAGCACC 721 CCTCCGAGGT TCTTCGGTCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt