Construct: ORF TRCN0000472334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009421.1_s317c1
- Derived from:
- ccsbBroadEn_03001
- DNA Barcode:
- GCGCGGATCTCATGCTTTGTAGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TUBG2 (27175)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472334
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27175 | TUBG2 | tubulin gamma 2 | NM_016437.3 | 100% | 100% | |
| 2 | human | 27175 | TUBG2 | tubulin gamma 2 | NM_001320509.2 | 98% | 98% | 398_424del |
| 3 | human | 7283 | TUBG1 | tubulin gamma 1 | NM_001070.5 | 94.6% | 97.7% | (many diffs) |
| 4 | human | 7283 | TUBG1 | tubulin gamma 1 | XM_024450904.1 | 79.3% | 81.9% | (many diffs) |
| 5 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450695.1 | 76.8% | 48.5% | 844_1102del;1417_1534del;1731_1761del |
| 6 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450694.1 | 75.6% | 47.8% | (many diffs) |
| 7 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450699.1 | 71.2% | 65.7% | (many diffs) |
| 8 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450696.1 | 70.9% | 55.7% | 398_424del;871_1129del;1449_1450ins190 |
| 9 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_011524614.3 | 66% | 64.9% | 0_1ins379;18_19ins80 |
| 10 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024453.2 | 66% | 64.9% | 0_1ins379;18_19ins80 |
| 11 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024454.2 | 60.7% | 60.7% | 0_1ins531 |
| 12 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024455.2 | 60.7% | 60.7% | 0_1ins531 |
| 13 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_017024456.2 | 60.7% | 60.7% | 0_1ins531 |
| 14 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450703.1 | 60.7% | 60.7% | 0_1ins531 |
| 15 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450697.1 | 50.7% | 24% | (many diffs) |
| 16 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450698.1 | 50.7% | 24% | (many diffs) |
| 17 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450700.1 | 39.2% | 24.1% | 0_1ins531;313_571del;891_892ins190 |
| 18 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450701.1 | 39.2% | 24.1% | 0_1ins531;313_571del;891_892ins190 |
| 19 | human | 27175 | TUBG2 | tubulin gamma 2 | XM_024450702.1 | 39.2% | 24.1% | 0_1ins531;313_571del;891_892ins190 |
| 20 | mouse | 103768 | Tubg2 | tubulin, gamma 2 | NM_134028.2 | 92% | 97.5% | (many diffs) |
| 21 | mouse | 103733 | Tubg1 | tubulin, gamma 1 | NM_134024.2 | 90.3% | 97.1% | (many diffs) |
| 22 | mouse | 103733 | Tubg1 | tubulin, gamma 1 | XM_017314211.1 | 68.5% | 74.4% | (many diffs) |
| 23 | mouse | 103768 | Tubg2 | tubulin, gamma 2 | XM_006531917.2 | 55.8% | 59.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1419
- ORF length:
- 1353
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc ccgggagatc atcaccctgc agctgggcca gtgcggcaac cagattgggt 121 tcgagttctg gaaacagctg tgcgccgagc atggtatcag ccccgagggc atcgtggagg 181 aattcgccac cgagggcact gaccgcaagg acgtcttttt ctaccaggca gacgatgagc 241 actacatccc ccgggccgtg ctgctggact tggaaccccg ggtgatccac tccatcctca 301 actcccccta tgccaagctc tacaacccag agaacatcta cctgtcggaa catggaggag 361 gagctggcaa caactgggcc agcggattct cccagggtga gaaaattcat gaagacatct 421 ttgacatcat agaccgagaa gcagatggaa gtgacagttt ggagggcttc gtgctgtgtc 481 actccatcgc tgggggtacg ggttctggcc tgggctccta cctcctggag cgactgaatg 541 acaggtaccc caagaagcta gtgcagactt attcagtgtt tccctaccag gacgagatga 601 gcgacgtagt ggttcagccc tacaattcac tcctgacact caagaggctg acgcagaacg 661 cagattgtgt ggtggtgctg gacaacacag ccctgaaccg gattgccaca gaccgcctgc 721 acatccagaa cccgtccttc tcccagatca accagctggt gtccaccatc atgtcggcca 781 gcaccaccac cctgcgctac cccggctaca tgaacaatga cctcatcggc ctcatcgcct 841 cgctcattcc caccccacgg ctccacttcc tcatgaccGG CTACACCCCG CTCACTACAG 901 ACCAGTCAGT GGCCAGCGTG AGGAAGACCA CGGTCCTGGA TGTCATGAGG CGGCTGCTGC 961 AGCCCAAGAA CGTGATGGTG TCCACAGGCC GAGACCGCCA GACCAACCAC TGCTACATCG 1021 CCATCCTCAA CATCATCCAG GGAGAGGTGG ACCCCACCCA GGTCCACAAG AGCCTGCAGA 1081 GGATCCGGGA ACGGAAGTTG GCCAACTTCA TCCCGTGGGG CCCCGCCAGC ATCCAGGTGG 1141 CCCTGTCGAG GAAGTCTCCC TACCTGCCCT CGGCCCACCG GGTCAGCGGG CTCATGATGG 1201 CCAACCACAC CAGCATCTCC TCGCTCTTTG AAAGTTCCTG CCAGCAGTTT GACAAGCTGC 1261 GGAAGCGGGA TGCCTTCCTC GAGCAGTTCC GTAAGGAGGA CATGTTCAAG GACAACTTTG 1321 ATGAGATGGA CAGGTCTAGG GAGGTTGTTC AGGAGCTCAT TGATGAGTAC CATGCGGCCA 1381 CCCAGCCAGA CTACATTTCC TGGGGCACCC AGGAGCAGTG CCCAACTTTC TTGTACAAAG 1441 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1501 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1561 ACGAGCGCGG ATCTCATGCT TTGTAGTAAC GCGTTAAGTC gacaatcaac ctctggatta 1621 caaaatttgt gaaagatt