Construct: ORF TRCN0000472384
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006740.1_s317c1
- Derived from:
- ccsbBroadEn_03329
- DNA Barcode:
- TTCCGCCGGAATGGTGCTAATATG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTFP1 (51537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472384
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51537 | MTFP1 | mitochondrial fission proce... | NM_016498.5 | 100% | 100% | |
| 2 | human | 51537 | MTFP1 | mitochondrial fission proce... | NM_001003704.3 | 41.5% | 42.3% | 193_194ins233;266_405del |
| 3 | mouse | 67900 | Mtfp1 | mitochondrial fission proce... | NM_026443.4 | 86.7% | 86.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 563
- ORF length:
- 498
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttgcatgtca gagccGCAGC CGCGGGGCGC AGAGCGCGAT CTCTACCGGG ACACGTGGGT 121 GCGATACCTG GGCTATGCCA ATGAGGTGGG CGAGGCTTTC CGCTCTCTTG TGCCAGCGGC 181 GGTGGTGTGG CTGAGCTATG GCGTGGCCAG CTCCTACGTG CTGGCGGATG CCATTGACAA 241 AGGCAAGAAG GCTGGAGAGG TGCCCAGCCC TGAAGCAGGC CGCAGCGCCA GGGTGACTGT 301 GGCTGTGGTG GACACCTTTG TATGGCAGGC TCTAGCCTCT GTGGCCATTC CGGGCTTCAC 361 CATCAACCGC GTGTGTGCTG CCTCTCTCTA TGTCCTGGGC ACTGCCACCC GCTGGCCCCT 421 GGCTGTCCGC AAGTGGACCA CCACCGCGCT TGGGCTGTTG ACCATCCCCA TCATTATCCA 481 CCCCATTGAC AGGTCGGTGG ATTTCCTCCT GGACTCCAGC CTGCGCAAGC TCTACCCAAC 541 AGTGGGGAAG CCCAGCTCCT CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 601 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 661 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT CCGCCGGAAT 721 GGTGCTAATA TGACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 781 tt