Construct: ORF TRCN0000472431
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004162.1_s317c1
- Derived from:
- ccsbBroadEn_12009
- DNA Barcode:
- AAGAACAACTGCTAGCAGAATCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMCN (51705)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472431
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51705 | EMCN | endomucin | XM_017008290.1 | 78% | 78% | 417_418ins117 |
| 2 | human | 51705 | EMCN | endomucin | NM_001159694.2 | 71.5% | 68.5% | (many diffs) |
| 3 | human | 51705 | EMCN | endomucin | NM_016242.4 | 68.1% | 68.1% | 415_663del |
| 4 | human | 51705 | EMCN | endomucin | XM_011532024.3 | 68.1% | 68.1% | 415_663del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 600
- ORF length:
- 534
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga actgcttcaa gtgaccattc tttttcttct gcccagtatt tgcagcagta 121 acagcacagg tgttttagag gcagctaata attcacttgt tgttactaca acaaaaccat 181 ctataacaac accaaacaca gaatcattac agaaaaatgt tgtcacacca acaactggaa 241 caactccTAA AGGAACAATC ACCAATGAAT TACTTAAAAT GTCTCTGATG TCAACAGCTA 301 CTTTTTTAAC AAGTAAAGAT GAAGGATTGA AAGCCACAAC CACTGATGTC AGGAAGAATG 361 ACTCCATCAT TTCAAACGTA ACAGTAACAA GTGTTACACT TCCAAATGCT GTTTCAACAT 421 TACAAAGTTC CAAACCCAAG ACTGAAACTC AGAGTTCAAT TAAAACAACA GAAATACCAG 481 GCACACCAGA AAATGGAAAT GATCAACCTC AGTCTGATAA AGAGAGCGTG AAGCTTCTTA 541 CCGTTAAGAC AATTTCTCAT GAGTCTGGTG AGCACTCTGC ACAAGGAAAA ACCAAGAACT 601 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 661 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 721 TTTATATATC TTGTGGAAAG GACGAAAGAA CAACTGCTAG CAGAATCGCA CGCGTTAAGT 781 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt