Construct: ORF TRCN0000472432
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005917.1_s317c1
- Derived from:
- ccsbBroadEn_08653
- DNA Barcode:
- TACCCGTAGACAACACAAGATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LGALS14 (56891)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472432
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 56891 | LGALS14 | galectin 14 | NM_020129.3 | 99.5% | 98.5% | 199T>C;381C>G |
| 2 | human | 56891 | LGALS14 | galectin 14 | NM_203471.2 | 81% | 78.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 483
- ORF length:
- 417
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc atcactaccc gtaccataca cactgcctgt ttccttgcct gttggttcgt 121 gcgtgataaT CACAGGGACA CCGATCCTCA CTTTTGTCAA GGACCCACAG CTGGAGGTGA 181 ATTTCTACAC TGGGATGGAT GAGGACTCAG ATATTGCTTT CCAATTCCGA CTGCACTTTG 241 GTCATCCTGC AATCATGAAC AGTCGTGTGT TTGGCATATG GAGATATGAG GAGAAATGCT 301 ACTATTTACC CTTTGAAGAT GGCAAACCAT TTGAGCTGTG CATCTATGTG CGTCACAAGG 361 AATACAAGGT AATGGTAAAT GGCCAACGCA TTTACAACTT TGCCCATCGA TTCCCGCCAG 421 CATCTGTGAA GATGCTGCAA GTCTTGAGAG ATATCTCCCT GACCAGAGTG CTTATCAGCG 481 ATTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 541 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 601 GGCTTTATAT ATCTTGTGGA AAGGACGATA CCCGTAGACA ACACAAGATC TCACGCGTTA 661 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt