Construct: ORF TRCN0000472441
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000119.1_s317c1
- Derived from:
- ccsbBroadEn_09394
- DNA Barcode:
- AGCCCATGGCTATGCCAAAGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PALM2 (114299)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472441
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 445815 | PALM2AKAP2 | PALM2 and AKAP2 fusion | NM_001037293.2 | 99.8% | 99.7% | 305A>G;459T>C |
| 2 | human | 445815 | PALM2AKAP2 | PALM2 and AKAP2 fusion | NM_053016.6 | 91.1% | 91% | (many diffs) |
| 3 | mouse | 242481 | Palm2 | paralemmin 2 | NM_172868.3 | 88.8% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gatggcagag gcggaattgc acaaggaaag gctgcaagcc atagcagaaa 121 aaagaaagag gcagactgaa atagaaggca agcgacaaca gcttgacgag cagatacttc 181 tgctgcagca ttccaagtcc aaagtgcttc gggagaaatg gctgctgcag ggcatacccg 241 ctggaactgc cgaagaggag gaagccagga ggcggcagtc tgaagaggat gagttcagag 301 tcaagcaact tgaagataac attcagaggc tggagcaaga aatacaaacg ctagaaagtg 361 aagagtcccg gatatctgcc aaagagcaaa tcatcctaga gaaactgaag gaaacagaaa 421 aatccttcaa ggactttcag aagggtttct ccagtacgga tggagctgtg tacgccatgg 481 aaattaatgt ggagaaagac aaacaaacag gagagaccaa gatcctctct acatctacca 541 ttggcccaga gggggtccat cagaaaggag tcaaagtcta tgatgatggt accaaagtag 601 tgtatgaggt gcgctcagga ggcaccgtag tagaaaatgg agtgcacaaa ttaagcacaa 661 aggatgtaga agagcttatt cagaaggctg gacaatcaag cttaggagga gggcacgtgt 721 ctgaaaggac tgtgattgca gatgggagcc TCAGCCATCC CAAGGAACAC ATGCTCTGCA 781 AAGAAGCTAA GTTAGAAATG GTACATAAGT CTAGGAAAGA CCATTCTTCC GGGAACCCAG 841 GGCAGCAGGC CCAAGCCCCC AGCGCTGCAG GGCCGGAGGC AAACTTGGAT CAGCCCGTCA 901 CCATGATTTT TATGGGCTAC CAAAATATCG AGGATGAAGA GGAGACGAAA AAGGTGCTAG 961 GCTATGATGA AACCATCAAG GCTGAATTGG TCCTCATTGA TGAAGATGAT GAGAAGTCAT 1021 TGAGGGAGAA GACAGTGACG GACGTGTCCA CTATTGACGG GAACGCGGCT GAGCTTGTGT 1081 CCGGGAGGCC GGTCTCAGAC ACCACAGAGC CCTCATCCCC AGAAGGGAAG GAAGAGAGCC 1141 TAGCTACAGA GCCAGCCCCA GGTACCCAAA AGAAAAAGCG CTGTCAATGC TGTGTTGTCA 1201 TGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGAAG CCCATGGCTA TGCCAAAGTG TTACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt