Construct: ORF TRCN0000472454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015924.1_s317c1
- Derived from:
- ccsbBroadEn_02419
- DNA Barcode:
- TGCCTCATACAATATACTAATATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDRG1 (10397)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472454
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001135242.2 | 100% | 100% | |
| 2 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_006096.4 | 100% | 100% | |
| 3 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | XM_011516791.1 | 95.8% | 95.8% | 536_586del |
| 4 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001258432.2 | 83.2% | 83.2% | 0_1ins198 |
| 5 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | NM_001258433.1 | 79.4% | 79.4% | 0_1ins243 |
| 6 | human | 10397 | NDRG1 | N-myc downstream regulated 1 | XM_011516792.2 | 52% | 52% | 0_1ins567 |
| 7 | mouse | 17988 | Ndrg1 | N-myc downstream regulated ... | NM_008681.2 | 86.3% | 93.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcgggagatg caggatgtag acctcgctga ggtgaagcct ttggtggaga 121 aaggggagac catcaccggc ctcctgcaag agtttgatgt ccaggagcag gacatcgaga 181 ctttacatgg ctctgttcac gtcacgctgt gtgggactcc caagggaaac cggcctgtca 241 tcctcaccta ccatgacatc ggcatgaacc acaaaacctg ctacaacccc ctcttcaact 301 acgaggacat gcaggagatc acccagcact ttgccgtctg ccacgtggac gcccctggcc 361 agcaggacgg cgcagcctcc ttccccgcag ggtacatgta cccctccatg gatcagctgg 421 ctgaaatgct tcctggagtc cttcaacagt ttgggctgaa aagcattatt ggcatgggaa 481 caggagcagg cgcctacatc ctaactcgat ttgctctaaa caaccctgag atggtggagg 541 gccttgtcct tatcaacgtg aacccttgtg cggaaggctg gatggactgg gccgcctcca 601 agatctcagg atggacccaa gctctgccgg acatggtggt gtcccacctt tttgggaagg 661 aagaaatgca gagtaacgtg gaagtggtcc acacctaccg ccagcacatt gtgaatgaca 721 tgaaccccgg caacctgcac ctgttcatca atgcctacaa cagccggcgc gacctggaga 781 ttgagcgacc aatgccggga acccacacag tcaccctgca gtgcccTGCT CTGTTGGTGG 841 TTGGGGACAG CTCGCCTGCA GTGGATGCCG TGGTGGAGTG CAACTCAAAA TTGGACCCAA 901 CAAAGACCAC TCTCCTCAAG ATGGCGGACT GTGGCGGCCT CCCGCAGATC TCCCAGCCGG 961 CCAAGCTCGC TGAGGCCTTC AAGTACTTCG TGCAGGGCAT GGGATACATG CCCTCGGCTA 1021 GCATGACCCG CCTGATGCGG TCCCGCACAG CCTCTGGTTC CAGCGTCACT TCTCTGGATG 1081 GCACCCGCAG CCGCTCCCAC ACCAGCGAGG GCACCCGAAG CCGCTCCCAC ACCAGCGAGG 1141 GCACCCGCAG CCGCTCGCAC ACCAGCGAGG GGGCCCACCT GGACATCACC CCCAACTCGG 1201 GTGCTGCTGG GAACAGCGCC GGGCCCAAGT CCATGGAGGT CTCCTGCTAC CCAACTTTCT 1261 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1321 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1381 GTGGAAAGGA CGATGCCTCA TACAATATAC TAATATCACG CGTTAAGTCg acaatcaacc 1441 tctggattac aaaatttgtg aaagatt