Construct: ORF TRCN0000472456
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017139.1_s317c1
- Derived from:
- ccsbBroadEn_12700
- DNA Barcode:
- ATGGTTACAAACCCCTGCGGAGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACAD10 (80724)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472456
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80724 | ACAD10 | acyl-CoA dehydrogenase fami... | NM_025247.6 | 22.3% | 22.2% | 1_144del;852_854delCCC;858_3177delinsA |
| 2 | human | 80724 | ACAD10 | acyl-CoA dehydrogenase fami... | NM_001136538.2 | 21.7% | 21.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cggagttctc attccttctc cagggagagt cgctgcagaa tgggaggtac 121 agaatcgtat cccttctgga actatattaa aggccttgat ggaaggtggt gaaaatgggc 181 cctggatgag atttatgaga gcagaaataa cagcagaggg ttttttacga gaatttggga 241 gactttgctc tgaaatgtta aagacctccG TGCCTGTGGA CTCATTTTTC TCTCTGTTGA 301 CCAGTGAGCG AGTGGCAAAG CAGTTCCCAG TGATGACTGA GGCCATAACT CAAATTCGGG 361 CAAAAGGTCT TCAGACTGCA GTCTTGAGCA ATAATTTTTA TCTTCCCAAC CAGAAAAGCT 421 TTTTGCCCCT GGACCGGAAA CAGTTTGATG TGATTGTGGA GTCCTGCATG GAAGGGATCT 481 GTAAGCCAGA CCCTAGGATC TACAAGCTGT GCTTGGAGCA GCTCGGCCTG CAGCCCTCTG 541 AGTCCATCTT TCTTGATGAC CTTGGAACAA ATCTAAAAGA AGCTGCCAGA CTTGGTATTC 601 ACACCATTAA GGTTAATGAC CCAGAGACTG CAGTAAAGGA ATTAGAAGCT CTCTTGGGTT 661 TTACATTGAG AGTAGGTGTT CCAAACACTC GGCCTGTGAA AAAGACGATG GAAATTCCGA 721 AAGATTCCTT GCAGAAGTAC CTCAAAGACT TACTGGGTAT CCAGACCACA GGATTATGCC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GAATGGTTAC AAACCCCTGC GGAGCTACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt