Construct: ORF TRCN0000472465

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006061.1_s317c1
Derived from:
ccsbBroadEn_01149
DNA Barcode:
ATTCCTAGATCCGTCTAAAAGCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PCBP1 (5093)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472465
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5093 PCBP1 poly(rC) binding protein 1 NM_006196.4 100% 100%
2 human 5094 PCBP2 poly(rC) binding protein 2 NM_001128913.1 77.6% 80.7% (many diffs)
3 human 5094 PCBP2 poly(rC) binding protein 2 NM_001098620.3 77% 79.6% (many diffs)
4 mouse 23983 Pcbp1 poly(rC) binding protein 1 NM_011865.4 94.1% 100% (many diffs)
5 mouse 18521 Pcbp2 poly(rC) binding protein 2 XM_006520584.3 77.3% 80.7% (many diffs)
6 mouse 18521 Pcbp2 poly(rC) binding protein 2 NM_001103166.1 76.7% 79.6% (many diffs)
7 mouse 18521 Pcbp2 poly(rC) binding protein 2 XM_017316500.1 76.5% 79.3% (many diffs)
8 mouse 18521 Pcbp2 poly(rC) binding protein 2 NM_001174073.1 73.9% 77.3% (many diffs)
9 mouse 18521 Pcbp2 poly(rC) binding protein 2 XM_006520585.3 73.9% 77.3% (many diffs)
10 mouse 18521 Pcbp2 poly(rC) binding protein 2 XM_017316501.1 73.8% 77.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1134
ORF length:
1068
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga tgccggtgtg actgaaagtg gactaaatgt gactctcacc attcggcttc
121ttatgcacgg aaaggaagta ggaagcatca ttgggaagaa aggggagtcg gttaagagga
181tccgcgagga gagtggcgcg cggatcaaca tctcggaggg gaattgtccg gagagaatca
241tcactctgac cggccccacc aatgccatct ttaaggcttt cgctatgatc atcgacaagc
301tggaggaaga tatcaacagc tccatgacca acagtaccgc ggccagcagg cccccggtca
361ccctgaggct ggtggtgccg gccacccagt gcggctccct gattgggaaa ggcgggtgta
421agatcaaaga gatccgcgag agtacggggg cgcaggtcca ggtggcgggg gatatgctgc
481ccaactccac cgagcgggcc atcaccatcg ctggcgtgcc gcagtctgtc accgagtgtg
541tcaagcagat ttgcctggtc atgctggaga cgctctccca gtctccgcaa gggagagtca
601tgaccattcc gtaccagccc atgccggcca gctccccagT CATCTGCGCG GGCGGCCAAG
661ATCGGTGCAG CGACGCTGCG GGCTACCCCC ATGCCACCCA TGACCTGGAG GGACCACCTC
721TAGATGCCTA CTCGATTCAA GGACAACACA CCATTTCTCC GCTCGATCTG GCCAAGCTGA
781ACCAGGTGGC AAGACAACAG TCTCACTTTG CCATGATGCA CGGCGGGACC GGATTCGCCG
841GAATTGACTC CAGCTCTCCA GAGGTGAAAG GCTATTGGGC AAGTTTGGAT GCATCTACTC
901AAACCACCCA TGAACTCACC ATTCCAAATA ACTTAATTGG CTGCATAATC GGGCGCCAAG
961GCGCCAACAT TAATGAGATC CGCCAGATGT CCGGGGCCCA GATCAAAATT GCCAACCCAG
1021TGGAAGGCTC CTCTGGTAGG CAGGTTACTA TCACTGGCTC TGCTGCCAGT ATTAGTCTGG
1081CCCAGTATCT AATCAATGCC AGGCTTTCCT CTGAGAAGGG CATGGGGTGC AGCTACCCAA
1141CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
1201ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
1261TATCTTGTGG AAAGGACGAA TTCCTAGATC CGTCTAAAAG CCCACGCGTT AAGTCgacaa
1321tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)