Construct: ORF TRCN0000472589
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009472.1_s317c1
- Derived from:
- ccsbBroadEn_08250
- DNA Barcode:
- AGTTTCTACGGGCCTTCGTGTCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CPA4 (51200)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472589
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51200 | CPA4 | carboxypeptidase A4 | NM_016352.3 | 99.9% | 100% | 1164C>T |
| 2 | human | 51200 | CPA4 | carboxypeptidase A4 | NM_001163446.1 | 92% | 92.1% | 285_286ins99;1065C>T |
| 3 | mouse | 71791 | Cpa4 | carboxypeptidase A4 | NM_027926.3 | 85% | 83.8% | (many diffs) |
| 4 | mouse | 71791 | Cpa4 | carboxypeptidase A4 | XM_017321750.1 | 76.2% | 75.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1329
- ORF length:
- 1263
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gtggatactg ttcattgggg cccttattgg gtccagcatc tgtggccaag 121 aaaaattttt tggggaccaa gttttgagga ttaatgtcag aaatggagac gagatcagca 181 aattgagtca actagtgaat tcaaacaact tgaagctcaa tttctggaaa tctccctcct 241 ccttcaatcg gcctgtggat gtcctggtcc catctgtcag tctgcaggca tttaaatcct 301 tcctgagatc ccagggctta gagtacgcag tgacaattga ggacctgcag gcccttttag 361 acaatgaaga tgatgaaatg caacacaatg aagggcaaga acggagcagt aataacttca 421 actacggggc ttaccattcc ctggaagcta tttaccacga gatggacaac attgccgcag 481 actttcctga cctggcgagg agggtgaaga ttggacattc gtttgaaaac cggccgatgt 541 atgtactgaa gttcagcact gggaaaggcg tgaggcggcc ggccgtttgg ctgaatgcag 601 gcatccattc ccgagagtgg atctcccagg ccactgcaat ctggacggca aggaagattg 661 tatctgatta ccagagggat ccagctatca cctccatctt ggagaaaatg gatattttct 721 tgttgcctgt ggccaatcct gatggatatg tgtatactca aactcaaaac cgattatgga 781 ggaagacgcg gtcccgaaat cctggaagct cctgcattgg tgctgaccca aatagaaact 841 ggaacgctag ttttgcagga aagggagcca gcgacaaccc ttgctccGAA GTGTACCATG 901 GACCCCACGC CAATTCGGAA GTGGAGGTGA AATCAGTGGT AGATTTCATC CAAAAACATG 961 GGAATTTCAA GGGCTTCATC GACCTGCACA GCTACTCGCA GCTGCTGATG TATCCATATG 1021 GGTACTCAGT CAAAAAGGCC CCAGATGCCG AGGAACTCGA CAAGGTGGCG AGGCTTGCGG 1081 CCAAAGCTCT GGCTTCTGTG TCGGGCACTG AGTACCAAGT GGGTCCCACC TGCACCACTG 1141 TCTATCCAGC TAGCGGGAGC AGCATCGACT GGGCGTATGA CAACGGCATC AAATTTGCAT 1201 TCACATTTGA GTTGAGAGAT ACCGGGACTT ATGGCTTCCT CCTGCCAGCT AACCAGATCA 1261 TCCCCACTGC AGAGGAGACG TGGCTGGGGC TGAAGACCAT CATGGAGCAT GTGCGGGACA 1321 ACCTCTACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1381 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1441 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTTTC TACGGGCCTT CGTGTCGTAC 1501 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt