Construct: ORF TRCN0000472604
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008768.1_s317c1
- Derived from:
- ccsbBroadEn_02206
- DNA Barcode:
- GATCTATGTTCTATTTGGTTGGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IER2 (9592)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472604
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9592 | IER2 | immediate early response 2 | NM_004907.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 735
- ORF length:
- 669
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agtgcagaaa gaggcacagc gcatcatgac cctgtcggtg tggaagatgt 121 atcactcccg catgcagcgc ggtggcctgc ggctgcaccg gagtctgcag ctgtcgctgg 181 tcatgcgcag cgcccgggag ctctacctct cggccaaggt ggaggccctc gagcccGAGG 241 TGTCGTTGCC GGCCGCCCTC CCCTCTGACC CTCGCCTGCA CCCGCCCCGA GAAGCCGAGT 301 CCACGGCCGA GACAGCGACC CCCGACGGTG AGCACCCGTT TCCGGAGCCA ATGGACACGC 361 AGGAGGCGCC GACAGCCGAG GAGACCTCCG CCTGCTGTGC CCCGCGCCCC GCCAAAGTCA 421 GCCGCAAACG ACGCAGCAGC AGCCTGAGCG ACGGCGGGGA CGCTGGACTG GTCCCGAGCA 481 AGAAAGCCCG TCTGGAAGAA AAGGAAGAAG AGGAGGGAGC GTCATCCGAA GTCGCCGATC 541 GCCTGCAGCC CCCTCCGGCG CAAGCGGAGG GCGCCTTTCC CAACCTGGCC CGCGTCCTGC 601 AGAGGCGCTT CTCCGGCCTC CTGAACTGCA GCCCCGCGGC CCCTCCGACG GCGCCGCCCG 661 CGTGCGAGGC AAAGCCCGCT TGCCGCCCGG CGGACAGCAT GCTCAACGTG CTCGTGCGGG 721 CCGTGGTGGC CTTCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GATCTATGTT CTATTTGGTT 901 GGTGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt