Construct: ORF TRCN0000472613
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009848.1_s317c1
- Derived from:
- ccsbBroadEn_06504
- DNA Barcode:
- CTATACTGCCCATCTAAAAGCGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT6A (3853)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472613
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3853 | KRT6A | keratin 6A | NM_005554.4 | 99.8% | 99.8% | 62A>G;483T>C;819T>C |
| 2 | human | 286887 | KRT6C | keratin 6C | NM_173086.5 | 97.8% | 98.2% | (many diffs) |
| 3 | human | 3854 | KRT6B | keratin 6B | NM_005555.4 | 97.4% | 98.4% | (many diffs) |
| 4 | mouse | 16687 | Krt6a | keratin 6A | NM_008476.3 | 85.7% | 86.5% | (many diffs) |
| 5 | mouse | 16688 | Krt6b | keratin 6B | NM_010669.2 | 85.5% | 85.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1758
- ORF length:
- 1692
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cagcacatcc accaccatca ggagccacag cagcagccgc cggggtttca 121 gtgccagctc agccaggctc cctggggtca gccgctctgg cttcagcagc gtctccgtgt 181 cccgctccag gggcagtggt ggcctgggtg gtgcatgtgg aggagctggc tttggcagcc 241 gcagtctgta tggcctgggg ggctccaaga ggatctccat tggagggggc agctgtgcca 301 tcagtggcgg ctatggcagc agagccggag gcagctatgg ctttggtggc gccgggagtg 361 gatttggttt cggtggtgga gccggcattg gctttggtct gggtggtgga gccggccttg 421 ctggtggctt tgggggccct ggcttccctg tgtgcccccc tggaggcatc caagaggtca 481 ccgtcaacca gagtctcctg actcccctca acctgcaaat cgatcccacc atccagcggg 541 tgcgggccga ggagcgtgaa cagatcaaga ccctcaacaa caagtttgcc tccttcatcg 601 acaaggtgcg gttcctggag cagcagaaca aggttctgga aacaaagtgg accctgctgc 661 aggagcaggg caccaagact gtgaggcaga acctggagcc gttgttcgag cagtacatca 721 acaacctcag gaggcagctg gacagcattg tcggggaacg gggccgcctg gactcagagc 781 tcagaggcat gcaggacctg gtggaggact tcaagaacaa atatgaggat gaaatcaaca 841 agcgcacagc agcagagaat gaatttgtga ctctgaagaa ggacgtggat gctgcctaca 901 tgaacaaggt tgaactgcaa gccaaggcag acactctcac agacgagatc aacttcctga 961 gagccttgta tgatgcagag ctgtcccaga tgcagaccca catctcagac acatctgtgg 1021 tgctgtccat ggacaacaac cgcaacctgg acctggacag catcatcgct gaggtcaagg 1081 cccaatatga ggagattgct cagagaagcc gggctgaggc tgagtcctgg taccagacca 1141 agtacgagga gctgcaggtc acagcaggca gacatgggga cgacctgcgc aacaccaagc 1201 aggagattgc tgagatcaac cgcatgatcc agaggctgag atctgagatc gaccacgtca 1261 agaagcagtg cgccaaccTG CAGGCCGCCA TTGCTGATGC TGAGCAGCGT GGGGAGATGG 1321 CCCTCAAGGA TGCCAAGAAC AAGCTGGAAG GGCTGGAGGA TGCCCTGCAG AAGGCCAAGC 1381 AGGACCTGGC CCGGCTGCTG AAGGAGTACC AGGAGCTGAT GAATGTCAAG CTGGCCCTGG 1441 ACGTGGAGAT CGCCACCTAC CGCAAGCTGC TGGAGGGTGA GGAGTGCAGG CTGAATGGCG 1501 AAGGCGTTGG ACAAGTCAAC ATCTCTGTGG TGCAGTCCAC CGTCTCCAGT GGCTATGGCG 1561 GTGCCAGTGG TGTCGGCAGT GGCTTAGGCC TGGGTGGAGG AAGCAGCTAC TCCTATGGCA 1621 GTGGTCTTGG CGTTGGAGGT GGCTTCAGTT CCAGCAGTGG CAGAGCCATT GGGGGTGGCC 1681 TCAGCTCTGT TGGAGGCGGC AGTTCCACCA TCAAGTACAC CACCACCTCC TCCTCCAGCA 1741 GGAAGAGCTA TAAGCACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1801 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1861 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTATACT GCCCATCTAA 1921 AAGCGTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt