Construct: ORF TRCN0000472617
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005163.1_s317c1
- Derived from:
- ccsbBroadEn_13259
- DNA Barcode:
- TATTACATTGTGCCCCCTTCTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC9B1 (150159)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472617
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NR_047515.2 | 44.9% | 1_469del;1124_1454del | |
| 2 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NM_001100874.3 | 41.6% | 36.2% | (many diffs) |
| 3 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NM_139173.4 | 38.5% | 33.4% | (many diffs) |
| 4 | human | 150159 | SLC9B1 | solute carrier family 9 mem... | NR_047513.2 | 37.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct tgagcatagt cttttcctca ggtgtggcct ccgtctttca cccaggctgg 121 agtgtggtgt cacgatctca gctcactgca aactctgcct cccgggttca agtgattctc 181 ctccctcagc cTCCCGAGTA CCTGGGACCA CAGGTGGTAT ACTTAATAAC GCCATAGCCT 241 CTATAAGGAA CGTATGTATT AGTCTGCTGG CAGGAATTGT TTTGGGATTT TTTGTTCGAT 301 ATTTTCCAAG TGAAGACCAG AAAAAACTTA CATTGAAGAG AGGATTCCTT GTTTTGACTA 361 TGTGTGTTTC TGCCGTCTTA GGCAGCCAAC GTATTGGTTT ACATGGATCT GGAGGATTAT 421 GCACACTAGT GTTGAGTTTC ATTGCAGGGA CAAAATGGTC CCAAGAAAAG ATGAAAGTCC 481 AAAAGATTAT TACGACTGTA TGGGATATTT TTCAACCACT TCTTTTTGGT TTAGTTGGAG 541 CAGAAGTATC TGTTTCATCG CTTGAATCAA ATATTGTTGG CATATCTGTT GCCACTCTAA 601 GTTTGGCATT ATGTGTTCGA ATTTTAACCA CATATCTATT GATGTGCTTT GCTGGTTTTA 661 GTTTTAAGGA GAAAATATTT ATTGCTTTAG CATGGATGCC CAAAGCTACA GTACAGAGAT 721 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGATATTA CATTGTGCCC CCTTCTTTAA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt