Construct: ORF TRCN0000472731
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007476.1_s317c1
- Derived from:
- ccsbBroadEn_10331
- DNA Barcode:
- GACATTGCACTCATCGTGCATATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPS2P32 (256355)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472731
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6187 | RPS2 | ribosomal protein S2 | NM_002952.4 | 53.5% | 23.6% | (many diffs) |
| 2 | human | 256355 | RPS2P32 | ribosomal protein S2 pseudo... | NR_026676.1 | 49.5% | 1_308del;825_1041del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 582
- ORF length:
- 516
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gatcaagtcc ctagaggaga tttatctctt ctcgctgccc atcaaggaat 121 ctgagatcat tgactttttc ctgggggcct ctctcaagga cgaggttttg aagattatgc 181 ctgtgcggaa ggagacccgc gccggccagc gcaccaggtt caaggcgttt gttgccatca 241 gggactacaa tggctacgct ggtcgggttg aagtgctccA AGGAGGTGGC CGCCGCCATC 301 CGCGGGGCCA TCATCCTGAC CAAGCTCTCC ATTGTCACCG TGCGCAGAGG CTACTGGGGG 361 AACAAGATCG GCAAGCTCCA CACCGTCCCT TGCAAGGTGA CAGGCCGCTG CGGCTCTGTG 421 CTGGTGCCCT TCATCCCCGC GCCCAGGGGC ACTGGCATCG TCTCAGCTCC TGTGCCCAAG 481 AAGCTGCTCA TGATAGCTGG TATCTACGAC TGCTACACCT CAGCCAGGGG CTGCACTGCC 541 ACCCTGGGCA ACTTCGCCAA CGCCACCTTG GATGCTGTCT CTACCCAACT TTCTTGTACA 601 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 661 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 721 AGGACGAGAC ATTGCACTCA TCGTGCATAT TACGCGTTAA GTCgacaatc aacctctgga 781 ttacaaaatt tgtgaaagat t