Construct: ORF TRCN0000472739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002209.1_s317c1
- Derived from:
- ccsbBroadEn_00586
- DNA Barcode:
- CACTAGCCGCGACAGTCATTATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNTB (2342)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2342 | FNTB | farnesyltransferase, CAAX b... | NM_002028.4 | 100% | 100% | |
| 2 | human | 100529261 | CHURC1-FNTB | CHURC1-FNTB readthrough | NM_001202558.2 | 89.3% | 89% | (many diffs) |
| 3 | human | 100529261 | CHURC1-FNTB | CHURC1-FNTB readthrough | NM_001202559.1 | 82.3% | 78.5% | (many diffs) |
| 4 | mouse | 110606 | Fntb | farnesyltransferase, CAAX b... | NM_145927.2 | 90.1% | 95.8% | (many diffs) |
| 5 | mouse | 110606 | Fntb | farnesyltransferase, CAAX b... | XM_006515404.2 | 50.2% | 53.3% | (many diffs) |
| 6 | mouse | 110606 | Fntb | farnesyltransferase, CAAX b... | XR_001780410.1 | 40.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1377
- ORF length:
- 1311
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttctccgagt tctttcacct actattgccc tccatcttcc tcccccgtct 121 ggtcagagcc gctgtacagt ctgaggcccg agcacgcgcg agagcggttg caggacgact 181 cggtggaaac agtcacgtcc atagaacagg caaaagtaga agaaaagatc caagaggtct 241 tcagttctta caagttcaac caccttgtac caaggcttgt tttgcagagg gagaagcact 301 tccattatct gaaaagaggc cttcgacaac tgacagatgc ctatgagtgt ctggatgcca 361 gccgcccatg gctctgctat tggatcctgc acagcttgga actgctagat gaacccatcc 421 cccagatagt ggctacagat gtgtgtcagt tcctggagct gtgtcagagc ccagaaggtg 481 gctttggagg aggacccggt cagtatccac accttgcacc cacatatgca gcagtcaatg 541 cattgtgcat cattggcacc gaggaggcct atgacatcat taacagagag aagcttcttc 601 agtatttgta ctccctgaag caacctgacg gctcctttct catgcatgtc ggaggtgagg 661 tggatgtgag aagcgcatac tgtgctgcct ccgtagcctc gctgaccaac atcatcactc 721 cagacctctt tgagggcact gctgaatgga tagcaaggtg tcagaactgg gaaggtggca 781 ttggcggggt accagggatg gaagcccatg gtggctatac cttctgtggc ctggccgcgc 841 tggtaatcct caagagggaa cgttccttga acttGAAGAG CTTATTACAA TGGGTGACAA 901 GCCGGCAGAT GCGATTTGAA GGAGGATTTC AGGGCCGCTG CAACAAGCTG GTGGATGGCT 961 GCTACTCCTT CTGGCAGGCG GGGCTCCTGC CCCTGCTCCA CCGCGCACTG CACGCCCAAG 1021 GTGACCCTGC CCTTAGCATG AGCCACTGGA TGTTCCATCA GCAGGCCCTG CAGGAGTACA 1081 TCCTGATGTG CTGCCAGTGC CCTGCGGGGG GGCTTCTGGA TAAACCTGGC AAGTCGCGTG 1141 ATTTCTACCA CACCTGCTAC TGCCTGAGCG GCCTGTCCAT AGCCCAGCAC TTCGGCAGCG 1201 GAGCCATGTT GCATGATGTG GTCCTGGGTG TGCCCGAAAA CGCTCTGCAG CCCACTCACC 1261 CAGTGTACAA CATTGGACCA GACAAGGTGA TCCAGGCCAC TACATACTTT CTACAGAAGC 1321 CAGTCCCAGG TTTTGAGGAG CTTAAGGATG AGACATCGGC AGAGCCTGCA ACCGACTACC 1381 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1441 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1501 ATATATCTTG TGGAAAGGAC GACACTAGCC GCGACAGTCA TTATCTACGC GTTAAGTCga 1561 caatcaacct ctggattaca aaatttgtga aagatt