Construct: ORF TRCN0000472744
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012837.1_s317c1
- Derived from:
- ccsbBroadEn_10706
- DNA Barcode:
- ATGCAGACACCAACGGGCCTGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CA12 (771)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472744
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 771 | CA12 | carbonic anhydrase 12 | NR_135511.2 | 6.9% | 1_3036del;3210G>C;3460_6112del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 513
- ORF length:
- 423
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGACGAAGCA ACATGAATTA GGGGGCCTCT 121 TGGCCTTGGT CCAAAATTGT CAATCAGAAA TGAACATAAA GGACTCCAGA GCAGTGGGAC 181 TGTCTGTCAA AAGACTCTGT ATATCTTTTG TGGATGAGTT TTGTGAGAGA ACAGAGAGAC 241 CATTGTACCT GGCACAAGGG CTCTTCATGA AAAGGGAGAC TTACTGGGAG GTGCAAGACA 301 GTGGCATTTC TCCTCTCCTC TTGCTGCTCA GCACAGCCCT GGATTGCAGC CCCGAGGCTG 361 AGACCAGACA AAGCCCGGGA GGCAGAAAGA TGCTCCAAGA ACCAACACTA TCAATGTCTT 421 TGCAAATCCT CACAGGATTC CTGTGGGTCC AGCTTTGGAA CTGGGAAACC TTTCTTCGGA 481 TCCGCACTCA TTCCACTGAT GCCAGCTGCC CCTGCCCAAC TTTCTTGTAC AAAGTGGTTG 541 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 601 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAT 661 GCAGACACCA ACGGGCCTGT GGACGCGTTA AGTCgacaat caacctctgg attacaaaat 721 ttgtgaaaga tt