Construct: ORF TRCN0000472779
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004050.1_s317c1
- Derived from:
- ccsbBroadEn_15362
- DNA Barcode:
- AGGGAATTTTAGCGATTCCCTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF4 (468)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472779
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 468 | ATF4 | activating transcription fa... | NM_001675.4 | 99.9% | 99.7% | 65A>C |
| 2 | human | 468 | ATF4 | activating transcription fa... | NM_182810.2 | 99.9% | 99.7% | 65A>C |
| 3 | human | 468 | ATF4 | activating transcription fa... | XM_017028807.2 | 99.9% | 99.7% | 65A>C |
| 4 | mouse | 11911 | Atf4 | activating transcription fa... | NM_001287180.1 | 85.5% | 85.7% | (many diffs) |
| 5 | mouse | 11911 | Atf4 | activating transcription fa... | NM_009716.3 | 85.5% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1119
- ORF length:
- 1053
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cgaaatgagc ttcctgagca gcgaggtgtt ggtgggggac ttgatgtccc 121 ccttcgaccc gtcgggtttg ggggctgaag aaagcctagg tctcttagat gattacctgg 181 aggtggccaa gcacttcaaa cctcatgggt tctccagcga caaggctaag gcgggctcct 241 ccgaatggct ggctgtggat gggttggtca gtccctccaa caacagcaag gaggatgcct 301 tctccgggac agattggatg ttggagaaaa tggatttgaa ggagttcgac ttggatgccc 361 tgttgggtat agatgacctg gaaaccatgc cagatgacct tctgaccacg ttggatgaca 421 cttgtgatct ctttgccccc ctagtccagg agactaataa gcagcccccc cagacggtga 481 acccaattgg ccatctccca gaaagtttaa caaaacccga ccaggttgcc cccttcacct 541 tcttacaacc tcttcccctt tccccagggg tcctgtcctc cacTCCAGAT CATTCCTTTA 601 GTTTAGAGCT GGGCAGTGAA GTGGATATCA CTGAAGGAGA TAGGAAGCCA GACTACACTG 661 CTTACGTTGC CATGATCCCT CAGTGCATAA AGGAGGAAGA CACCCCTTCA GATAATGATA 721 GTGGCATCTG TATGAGCCCA GAGTCCTATC TGGGGTCTCC TCAGCACAGC CCCTCTACCA 781 GGGGCTCTCC AAATAGGAGC CTCCCATCTC CAGGTGTTCT CTGTGGGTCT GCCCGTCCCA 841 AACCTTACGA TCCTCCTGGA GAGAAGATGG TAGCAGCAAA AGTAAAGGGT GAGAAACTGG 901 ATAAGAAGCT GAAAAAAATG GAGCAAAACA AGACAGCAGC CACTAGGTAC CGCCAGAAGA 961 AGAGGGCGGA GCAGGAGGCT CTTACTGGTG AGTGCAAAGA GCTGGAAAAG AAGAACGAGG 1021 CTCTAAAAGA GAGGGCGGAT TCCCTGGCCA AGGAGATCCA GTACCTGAAA GATTTGATAG 1081 AAGAGGTCCG CAAGGCAAGG GGGAAGAAAA GGGTCCCCTA CCCAACTTTC TTGTACAAAG 1141 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1201 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1261 ACGAAGGGAA TTTTAGCGAT TCCCTTACAC GCGTTAAGTC gacaatcaac ctctggatta 1321 caaaatttgt gaaagatt