Construct: ORF TRCN0000472784

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF019032.3_s317c1
Derived from:
ccsbBroadEn_14554
DNA Barcode:
GCCCACGCTACAATGCATCCTTGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BTK (695)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000472784
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 695 BTK Bruton tyrosine kinase NM_000061.2 100% 100%
2 human 695 BTK Bruton tyrosine kinase NM_001287344.1 95% 95% 1_102del
3 human 695 BTK Bruton tyrosine kinase NM_001287345.1 73.2% 73.2% 1033_1034ins528
4 mouse 12229 Btk Bruton agammaglobulinemia t... NM_013482.2 91.5% 98.3% (many diffs)
5 mouse 12229 Btk Bruton agammaglobulinemia t... XM_006528483.3 91.5% 98.3% (many diffs)
6 mouse 12229 Btk Bruton agammaglobulinemia t... XM_006528484.3 91.5% 98.3% (many diffs)
7 mouse 12229 Btk Bruton agammaglobulinemia t... XM_006528485.3 91.5% 98.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2046
ORF length:
1977
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat ggccgcagtg attctggaga gcatctttct gaagcgatcc caacagaaaa
121agaaaacatc acctctaaac ttcaagaagc gcctgtttct cttgaccgtg cacaaactct
181cctactatga gtatgacttt gaacgtggga gaagaggcag taagaagggt tcaatagatg
241ttgagaagat cacttgtgtt gaaacagtgg ttcctgaaaa aaatcctcct ccagaaagac
301agattccgag aagaggtgaa gagtccagtg aaatggagca aatttcaatc attgaaaggt
361tcccttatcc cttccaggtt gtatatgatg aagggcctct ctacgtcttc tccccaactg
421aagaactaag gaagcggtgg attcaccagc tcaaaaacgt aatccggtac aacagtgatc
481tggttcagaa atatcaccct tgcttctgga tcgatgggca gtatctctgc tgctctcaga
541cagccaaaaa tgctatgggc tgccaaattt tggagaacag gaatggaagc ttaaaacctg
601ggagttctca ccggaagaca aaaaagcctc ttcccccaac gcctgaggag gaccagatct
661tgaaaaagcc actaccgcct gagccagcag cagcaccagt ctccacaagt gagctgaaaa
721aggttgtggc cctttatgat tacatgccaa tgaatgcaaa tgatctacag ctgcggaagg
781gtgatgaata ttttatcttg gaggaaagca acttaccatg gtggagagca cgagataaaa
841atgggcagga aggctacatt cctagtaact atgtcactga agcagaagac tccatagaaa
901tgtatgagtg gtattccaaa cacatgactc ggagtcaggc tgagcaactg ctaaagcaag
961aggggaaaga aggaggtttc attgtcagag actccagcaa agctggcaaa tatacagtgt
1021ctgtgtttgc taaatccaca ggggaccctc aaggggtgat acgtcattat gttgtgtgtt
1081ccacacctca gagccagtat tacctggctg agaagcacct tttcagcacc atccctgagc
1141tcattaacta ccatcagcac aactctgcag gactcatatc caggctcaaa tatccagtgt
1201ctcaacaaaa caagaatgca ccttccactg caggcctggg atacggatca tgggaaattg
1261atccaaagga cctgaccttc ttgaaggagc tggggactgg acaatttggg gtagtgaagt
1321atgggaaatg gagaggccag tacgacgtgg ccatcaagat gatcaaagaa ggctccatgt
1381ctgaagatga attcattgaa gaagccaaag tcatgatgaa tctttcccat gagaagctgg
1441tgcagttgta tggcgtctgc accaagcagc gccccatctt catcatcact gagtacatgg
1501ccaatggctg cctcctgaac tacctgaggg agatgcgcca ccgcttccag actcagcagc
1561tgctagagat gtgcaaggat gtctgtgaag ccatggaata cctggagtca aagcagttcc
1621TTCACCGAGA CCTGGCAGCT CGAAACTGTT TGGTAAACGA TCAAGGAGTT GTTAAAGTAT
1681CTGATTTCGG CCTGTCCAGG TATGTCCTGG ATGATGAATA CACAAGCTCA GTAGGCTCCA
1741AATTTCCAGT CCGGTGGTCC CCACCGGAAG TCCTGATGTA TAGCAAGTTC AGCAGCAAAT
1801CTGACATTTG GGCTTTTGGG GTTTTGATGT GGGAAATTTA CTCCCTGGGG AAGATGCCAT
1861ATGAGAGATT TACTAACAGT GAGACTGCTG AACACATTGC CCAAGGCCTA CGTCTCTACA
1921GGCCTCATCT GGCTTCAGAG AAGGTATATA CCATCATGTA CAGTTGCTGG CATGAGAAAG
1981CAGATGAGCG TCCCACTTTC AAAATTCTTC TGAGCAATAT TCTAGATGTC ATGGATGAAG
2041AATCCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
2101TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
2161GGCTTTATAT ATCTTGTGGA AAGGACGAGC CCACGCTACA ATGCATCCTT GAACGCGTTA
2221AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)