Construct: ORF TRCN0000472818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012901.1_s317c1
- Derived from:
- ccsbBroadEn_10946
- DNA Barcode:
- CCTTCGCACTAAATTATAGCGATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIG3 (3980)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472818
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3980 | LIG3 | DNA ligase 3 | NM_002311.4 | 31.4% | 31.1% | (many diffs) |
| 2 | human | 3980 | LIG3 | DNA ligase 3 | XM_006721896.3 | 31.1% | 30.8% | (many diffs) |
| 3 | human | 3980 | LIG3 | DNA ligase 3 | NM_013975.4 | 29.5% | 29.3% | (many diffs) |
| 4 | human | 3980 | LIG3 | DNA ligase 3 | XM_017024624.1 | 29.5% | 29.3% | (many diffs) |
| 5 | human | 3980 | LIG3 | DNA ligase 3 | XM_005257970.4 | 29.2% | 29% | (many diffs) |
| 6 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532353.3 | 25.4% | 24.8% | (many diffs) |
| 7 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_001291246.1 | 25.3% | 24.7% | (many diffs) |
| 8 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_001291247.1 | 25.3% | 24.7% | (many diffs) |
| 9 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_001291245.1 | 25.3% | 24.7% | (many diffs) |
| 10 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | NM_010716.3 | 25.3% | 24.7% | (many diffs) |
| 11 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532350.3 | 25.3% | 24.7% | (many diffs) |
| 12 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532348.3 | 24.1% | 23.4% | (many diffs) |
| 13 | mouse | 16882 | Lig3 | ligase III, DNA, ATP-dependent | XM_006532347.3 | 24% | 23.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tttggctttc aagatcttct ttccacaaac cctccgtgca ctcagccgaa 121 aagaactgtg cctattccga aaacatcact ggcgtgatgt aagacaattc agccagtggt 181 cagaaacaga tctgcttcat ggacatcccc tcttcctgag aagaaagcct gttctatcat 241 tccagggaag ccatctaaga tcacgtgcca cctaccttgt tttcttgcca gggttgcatg 301 tgggactctg cagtggcccc tgtgagatgg ctgagcaacg gttctgtgtg gactatgcca 361 agcgtggcac agctggctgc aaaaaatgca aggaaaagat tgtgaagggc gtatgccgaa 421 ttggcaaagt ggtgcccaat cccttctcag agtctggggg tgatatgaaa gagtggtacc 481 acattaaatg catgtttgag aaactagagc gggcccgggC CACCACAAAA AAAATCGAGG 541 ACCTCACAGA GCTGGAAGGC TGGGAAGAGC TGGAAGATAA TGAGAAGGAA CAGATAACCC 601 AGCACATTGC AGATCTGTCT TCTAAGGCAG CAGGTACACC AAAGAAGAAA GCTGTTGTCC 661 AGGCTAAGTT GACAACCACT GGCCAGGTGA CTTCTCCAGT GAAAGGCGCC TCATTTGTCA 721 CCAGTACCAA TCCCCGGAAA TTTTCTGGCT TTTCAGCCAA GCCCAACAAC TCTGGGGAAG 781 CCCCCTCGAG CCCCACCCCT AAGAGAAGTC TGTCTTCAAG CAAATGTGAC CCCAGGCATA 841 AGGACTGTCT GCTACGGGAG TTTCGAAAGT TATGCGCCAT GGTGGCCGAT AATCCTAGCT 901 ACAACACGAA GACCCAGATC ATCCAGGACT TCCTTCGGAA AGGCTCAGCA GGAGGTGTGG 961 CATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGACC TTCGCACTAA ATTATAGCGA TTACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt