Construct: ORF TRCN0000472872
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008659.1_s317c1
- Derived from:
- ccsbBroadEn_00926
- DNA Barcode:
- CCAGCCCTTTCGTCCATCGTCGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LAMA4 (3910)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472872
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3910 | LAMA4 | laminin subunit alpha 4 | NM_001105208.2 | 100% | 100% | |
| 2 | human | 3910 | LAMA4 | laminin subunit alpha 4 | NM_001105209.2 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 426
- ORF length:
- 360
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGC TTTGAGCTCA GCCTGGCGCT CGGTTCTGCC TCTGTGGCTC CTCTGGAGCG 121 CTGCCTGCTC CCGCGCCGCG TCCGGGGACG ACAACGCTTT TCCTTTTGAC ATTGAAGGGA 181 GCTCAGCGGT TGGCAGGCAA GACCCGCCTG AGACGAGCGA ACCCCGCGTG GCTCTGGGAC 241 GCCTGCCGCC TGCGGCCGAG GTACAGTGTC CCTGCCATTG CCACCCTGCT GGGGCACCTG 301 CGCCCCCGCG GGCTGTGCCA CACTCGTCCT TCTCTCTCTC TCCGCCTCTT TCCTCTCCCC 361 AGTGCCTTGA GAGTTTCACC TGGGCTAGGT CAGTTCGGAA ACTTGAAATA AAGAGTTTTC 421 CTTTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 481 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 541 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCAGCCCTT TCGTCCATCG TCGTTACGCG 601 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt